Flow cytometric analysis of the binding of anti-Rh and anti-Rh-related MAbs to K562/RhD and K562/Lu stable clones
| Antibody . | Directed against . | K562/RhD MFI . | K562/Lu control MFI . | MFI ratio, K562/RhD over K562/Lu . |
|---|---|---|---|---|
| M. control | 2.9 | 2.3 | 1.2 | |
| BRIC69 | Rh polypeptides | 51.6 | 16.9 | 3.7 |
| BS56 | LW | 15.2 | 8.3 | 1.8 |
| 6H9 | CD47 | 146.4 | 81.8 | 1.8 |
| 2D10 | RhAG | 34.3 | 39.4 | 0.9 |
| H. control | 3.4 | 2.8 | 1.2 | |
| F5 | D | 38.9 | 7.3 | 5.3 |
| LOR-15C9 | D | 30.2 | 5.5 | 5.8 |
| RegA | epD1 | 6.2 | 2.2 | 2.7 |
| 5C8 | epD2 | 17.0 | 3.9 | 4.4 |
| 75 | epD3 | 27.5 | 8.0 | 3.4 |
| H27 | epD4 | 12.3 | 2.8 | 4.3 |
| Fom1 | epD5 | 3.6 | 1.9 | 1.9 |
| FogB | epD6 | 7.2 | 3.0 | 2.4 |
| RUM1 | epD7 | 38.1 | 9.8 | 3.9 |
| 105 | epD8 | 23.7 | 4.1 | 5.7 |
| MS26 | epD9 | 31.8 | 5.1 | 6.2 |
| RaE11 | c | 8.8 | 8.9 | 1.0 |
| Diagast IgM | C | 3.0 | 2.4 | 1.2 |
| MCG8 | E | 4.4 | 7.5 | 0.6 |
| H2G11 | G | 11.3 | 3.8 | 3.0 |
| Antibody . | Directed against . | K562/RhD MFI . | K562/Lu control MFI . | MFI ratio, K562/RhD over K562/Lu . |
|---|---|---|---|---|
| M. control | 2.9 | 2.3 | 1.2 | |
| BRIC69 | Rh polypeptides | 51.6 | 16.9 | 3.7 |
| BS56 | LW | 15.2 | 8.3 | 1.8 |
| 6H9 | CD47 | 146.4 | 81.8 | 1.8 |
| 2D10 | RhAG | 34.3 | 39.4 | 0.9 |
| H. control | 3.4 | 2.8 | 1.2 | |
| F5 | D | 38.9 | 7.3 | 5.3 |
| LOR-15C9 | D | 30.2 | 5.5 | 5.8 |
| RegA | epD1 | 6.2 | 2.2 | 2.7 |
| 5C8 | epD2 | 17.0 | 3.9 | 4.4 |
| 75 | epD3 | 27.5 | 8.0 | 3.4 |
| H27 | epD4 | 12.3 | 2.8 | 4.3 |
| Fom1 | epD5 | 3.6 | 1.9 | 1.9 |
| FogB | epD6 | 7.2 | 3.0 | 2.4 |
| RUM1 | epD7 | 38.1 | 9.8 | 3.9 |
| 105 | epD8 | 23.7 | 4.1 | 5.7 |
| MS26 | epD9 | 31.8 | 5.1 | 6.2 |
| RaE11 | c | 8.8 | 8.9 | 1.0 |
| Diagast IgM | C | 3.0 | 2.4 | 1.2 |
| MCG8 | E | 4.4 | 7.5 | 0.6 |
| H2G11 | G | 11.3 | 3.8 | 3.0 |
MFI indicates mean fluorescence intensity; M. control and H. control refer to murine and human isotype controls, respectively.