Sequences of the primers used
| Primers . | Direction . | Exon . |
|---|---|---|
| cDNA | ||
| 5′-TCACCCAGGGCTCCTGTAGCTCA-3′ | Sense | |
| 5′-CCGGGAGCGTCCTCCCTGTGTCCG-3′ | Antisense | |
| 5′-AGCAGATGCACGTCCTCGATACC-3′ | Sense | |
| 5′-TCGCCCTCCTCCTCATCTGAGCT-3 | Antisense | |
| Genomic DNA | ||
| 5′-TCACCCAGGGCTCCTGTAGCTCA-3′ | Sense | Exon 1 |
| 5′-TCGTGGGCAGGCAGGCAGGCGTCC-3′ | Antisense | Exon 1 |
| 5′-TGATGGGGACCAGCGTGCGGGGT-3′ | Sense | Exon 2 |
| 5′-TCTCTCCAGACCCCAGGACCAGGC-3′ | Antisense | Exon 2 |
| 5′-ACTATAACCTTTTCCTGCCCTGCC-3′ | Sense | Exon 3 |
| 5′-AGCCTGTGTCAGCTTCCTGTGAAG-3′ | Antisense | Exon 3 |
| 5′-TGCACTCCTTCTCTCCCCAGCTGACC-3′ | Sense | Exon 4 |
| 5′-ACACACACGCGACTCGGTGGCGTG-3′ | Antisense | Exon 4 |
| 5′-CCGGGCCCCTGTGCGTACGCAAGG-3′ | Sense | Exon 5 |
| 5′-CCGGGAGCGTCCTCCCTGTGTCCG-3′ | Antisense | Exon 5 |
| Primers . | Direction . | Exon . |
|---|---|---|
| cDNA | ||
| 5′-TCACCCAGGGCTCCTGTAGCTCA-3′ | Sense | |
| 5′-CCGGGAGCGTCCTCCCTGTGTCCG-3′ | Antisense | |
| 5′-AGCAGATGCACGTCCTCGATACC-3′ | Sense | |
| 5′-TCGCCCTCCTCCTCATCTGAGCT-3 | Antisense | |
| Genomic DNA | ||
| 5′-TCACCCAGGGCTCCTGTAGCTCA-3′ | Sense | Exon 1 |
| 5′-TCGTGGGCAGGCAGGCAGGCGTCC-3′ | Antisense | Exon 1 |
| 5′-TGATGGGGACCAGCGTGCGGGGT-3′ | Sense | Exon 2 |
| 5′-TCTCTCCAGACCCCAGGACCAGGC-3′ | Antisense | Exon 2 |
| 5′-ACTATAACCTTTTCCTGCCCTGCC-3′ | Sense | Exon 3 |
| 5′-AGCCTGTGTCAGCTTCCTGTGAAG-3′ | Antisense | Exon 3 |
| 5′-TGCACTCCTTCTCTCCCCAGCTGACC-3′ | Sense | Exon 4 |
| 5′-ACACACACGCGACTCGGTGGCGTG-3′ | Antisense | Exon 4 |
| 5′-CCGGGCCCCTGTGCGTACGCAAGG-3′ | Sense | Exon 5 |
| 5′-CCGGGAGCGTCCTCCCTGTGTCCG-3′ | Antisense | Exon 5 |
In order to analyze DNA, exon-specific genomic primer pairs were designed (GenBank accession numbers AC019059 and AC018410). One cDNA primer pair (the first pair) was designed to amplify the entire coding region of the human PU.1 cDNA, including 143 bp of the 5′UTR and 66 bp of the 3′UTR (GenBank accession number X52056). The second two cDNA primers correspond to sequences in the middle of the coding region and were used to sequence these amplified PCR products.