C-kit primers used in this study
| Primer . | Sequence . | Annealing temp, °C . | MgCl2concentration, mM . | Length, bp . |
|---|---|---|---|---|
| External | ||||
| c-kit–R1 | 5′-GCTGTGGTAGAGATCCCAT-3′ | 50 | 2 | 469 |
| 5′-CGCAATTTCACAGAAAACTC-3′ | ||||
| c-kit–R2 | 5′-TATTCACAGAGACTTGGCAG-3′ | 50 | 2.5 | 420 |
| 5′-ATTTCTCCTGCTGTGACCTT-3′ | ||||
| Nested | ||||
| c-kit–R1 | 5′-ATTGTAGAGCAAATCCATCCCC-3′ | 60 | 2 | 308 |
| 5′-GCCCCTGTTTCATACTGACCA-3′ | ||||
| c-kit–R2 | 5′-CCTCCTTACTCATGGTCGGATC-3′ | 60 | 2.5 | 292 |
| 5′-CTGTTTCCTTCACATGCCCC-3′ |
| Primer . | Sequence . | Annealing temp, °C . | MgCl2concentration, mM . | Length, bp . |
|---|---|---|---|---|
| External | ||||
| c-kit–R1 | 5′-GCTGTGGTAGAGATCCCAT-3′ | 50 | 2 | 469 |
| 5′-CGCAATTTCACAGAAAACTC-3′ | ||||
| c-kit–R2 | 5′-TATTCACAGAGACTTGGCAG-3′ | 50 | 2.5 | 420 |
| 5′-ATTTCTCCTGCTGTGACCTT-3′ | ||||
| Nested | ||||
| c-kit–R1 | 5′-ATTGTAGAGCAAATCCATCCCC-3′ | 60 | 2 | 308 |
| 5′-GCCCCTGTTTCATACTGACCA-3′ | ||||
| c-kit–R2 | 5′-CCTCCTTACTCATGGTCGGATC-3′ | 60 | 2.5 | 292 |
| 5′-CTGTTTCCTTCACATGCCCC-3′ |