Activity/antigen levels of the recombinant proteins
| Recombinant protein . | ELISA (μg/mL) . | PTase assay (μg/mL) . | Ratio Ptase/ELISA . |
|---|---|---|---|
| WT | 0.992 | 1.97 | 1.99 |
| 306G | 2.68 | 4.5 | 1.95 |
| 306T | 4.39 | 9.3 | 2.2 |
| 306Q | 2.7 | 4.6 | 1.88 |
| 506Q | 2.9 | 4.9 | 1.78 |
| 506Q/679Q | 3.2 | 6.7 | 2 |
| 306Q/679Q | 0.8 | 1.2 | 1.5 |
| 306Q/506Q/679Q | 6.8 | 10.86 | 1.6 |
| Recombinant protein . | ELISA (μg/mL) . | PTase assay (μg/mL) . | Ratio Ptase/ELISA . |
|---|---|---|---|
| WT | 0.992 | 1.97 | 1.99 |
| 306G | 2.68 | 4.5 | 1.95 |
| 306T | 4.39 | 9.3 | 2.2 |
| 306Q | 2.7 | 4.6 | 1.88 |
| 506Q | 2.9 | 4.9 | 1.78 |
| 506Q/679Q | 3.2 | 6.7 | 2 |
| 306Q/679Q | 0.8 | 1.2 | 1.5 |
| 306Q/506Q/679Q | 6.8 | 10.86 | 1.6 |
PTase assay and ELISA were performed as described in “Materials and methods,” and a ratio between the results from the 2 assays was calculated.