Dissociation constants for Spα:Spβ peptide complexes in samples containing native and substituted Spα 1-156 peptides and native Spβ1898-2083 peptides
| Peptide . | Kd(μM) . | Standard error . |
|---|---|---|
| Arg28/Arg45 | 0.93 | + 1.25 |
| − 0.53 | ||
| Arg45Thr | 6.5 | + 9.0 |
| − 3.8 | ||
| Arg45Ser | 44 | + 20 |
| − 14 | ||
| Arg28Ser | 59 | + 30 |
| − 20 |
| Peptide . | Kd(μM) . | Standard error . |
|---|---|---|
| Arg28/Arg45 | 0.93 | + 1.25 |
| − 0.53 | ||
| Arg45Thr | 6.5 | + 9.0 |
| − 3.8 | ||
| Arg45Ser | 44 | + 20 |
| − 14 | ||
| Arg28Ser | 59 | + 30 |
| − 20 |
Kd values were calculated as described in the “Materials and methods” section, using data at 7, 12, and 30 μM; uncertainties are asymmetric because Kd cannot be less than zero.