Effect of treatment with anti–LFA-1 antibodies (α–LFA-1) and G-CSF on blood cell counts
| Treatment . | White blood cells, × 109/L . | Neutrophils, × 109/L . | Platelets, × 109/L . |
|---|---|---|---|
| Saline | 8.3 ± 1.7 | 2.0 ± 0.7 | 1068 ± 279 |
| Saline | |||
| G-CSF 2 d, 2.5 μg/d | 9.8 ± 2.5 | 2.8 ± 1.6 | 738 ± 84 |
| α–LFA-1 | |||
| G-CSF 2 d, 2.5 μg/d | 9.3 ± 1.7 | 2.6 ± 0.5 | 309 ± 58* |
| Saline | |||
| G-CSF 1 d, 5 μg/d | 13.3 ± 3.5 | 7.0 ± 2.0 | 610 ± 367 |
| α–LFA-1 | |||
| G-CSF 1 d, 5 μg/d | 8.2 ± 1.6 | 3.3 ± 0.4 | 281 ± 85* |
| Saline | |||
| G-CSF 2 d, 5 μg/d | 12.4 ± 3.0 | 4.6 ± 2.0 | 936 ± 244 |
| α–LFA-1 | |||
| G-CSF 2 d, 5 μg/d | 8.9 ± 1.6 | 3.2 ± 1.8 | 634 ± 111* |
| Saline | |||
| G-CSF 3 d, 5 μg/d | 10.5 ± 3.2 | 3.4 ± 1.1 | 709 ± 180 |
| α–LFA-1 | |||
| G-CSF 3 d, 5 μg/d | 8.4 ± 1.8 | 3.6 ± 1.1 | 351 ± 313* |
| Saline | |||
| G-CSF 5 d, 5 μg/d | 19.6 ± 2.8 | 12.5 ± 2.3 | 705 ± 89 |
| α–LFA-1 | |||
| G-CSF 5 d, 5 μg/d | 22.8 ± 6.3 | 16.3 ± 4.1 | 922 ± 313 |
| Treatment . | White blood cells, × 109/L . | Neutrophils, × 109/L . | Platelets, × 109/L . |
|---|---|---|---|
| Saline | 8.3 ± 1.7 | 2.0 ± 0.7 | 1068 ± 279 |
| Saline | |||
| G-CSF 2 d, 2.5 μg/d | 9.8 ± 2.5 | 2.8 ± 1.6 | 738 ± 84 |
| α–LFA-1 | |||
| G-CSF 2 d, 2.5 μg/d | 9.3 ± 1.7 | 2.6 ± 0.5 | 309 ± 58* |
| Saline | |||
| G-CSF 1 d, 5 μg/d | 13.3 ± 3.5 | 7.0 ± 2.0 | 610 ± 367 |
| α–LFA-1 | |||
| G-CSF 1 d, 5 μg/d | 8.2 ± 1.6 | 3.3 ± 0.4 | 281 ± 85* |
| Saline | |||
| G-CSF 2 d, 5 μg/d | 12.4 ± 3.0 | 4.6 ± 2.0 | 936 ± 244 |
| α–LFA-1 | |||
| G-CSF 2 d, 5 μg/d | 8.9 ± 1.6 | 3.2 ± 1.8 | 634 ± 111* |
| Saline | |||
| G-CSF 3 d, 5 μg/d | 10.5 ± 3.2 | 3.4 ± 1.1 | 709 ± 180 |
| α–LFA-1 | |||
| G-CSF 3 d, 5 μg/d | 8.4 ± 1.8 | 3.6 ± 1.1 | 351 ± 313* |
| Saline | |||
| G-CSF 5 d, 5 μg/d | 19.6 ± 2.8 | 12.5 ± 2.3 | 705 ± 89 |
| α–LFA-1 | |||
| G-CSF 5 d, 5 μg/d | 22.8 ± 6.3 | 16.3 ± 4.1 | 922 ± 313 |
On day 0, mice were treated with a single intraperitoneal injection of 100 μg antibody or saline. Twenty-four hours later, G-CSF was administered as a once-daily injection. Twenty-four hours after the last injection of G-CSF, mice were killed and blood was obtained by a cardiac puncture. Results are expressed as mean ± SD (n = 3-18, 2-3 experiments).
P < .05 compared with the saline-pretreated controls.