Cytotoxicity of f-L-DOX to cultured leukemia cells: IC50 (μM) values for cells incubated in media containing 1 mM folic acid
| Leukemia cell line . | KG-1 . | KG-1a . | L1210JF . | L1210 . | ||
|---|---|---|---|---|---|---|
| FR expression status . | FR-β (+) . | FR (−) . | FR-α (+) . | FR (−) . | ||
| Pre-exposure to ATRA . | − . | + . | − . | + . | − . | − . |
| Free DOX | 0.67 | 0.68 | 0.63 | 0.62 | 0.87 | 0.93 |
| f-L-DOX | 11 | 5.4 | 21 | 19 | 12 | 29 |
| Nontargeted L-DOX | 27 | 29 | 23 | 20 | 35 | 31 |
| Leukemia cell line . | KG-1 . | KG-1a . | L1210JF . | L1210 . | ||
|---|---|---|---|---|---|---|
| FR expression status . | FR-β (+) . | FR (−) . | FR-α (+) . | FR (−) . | ||
| Pre-exposure to ATRA . | − . | + . | − . | + . | − . | − . |
| Free DOX | 0.67 | 0.68 | 0.63 | 0.62 | 0.87 | 0.93 |
| f-L-DOX | 11 | 5.4 | 21 | 19 | 12 | 29 |
| Nontargeted L-DOX | 27 | 29 | 23 | 20 | 35 | 31 |
Assays were repeated twice. Within each assay, cells were treated in quadruplicate. The error within each group was less than 20%.