Cytotoxicity of f-L-DOX to cultured leukemia cells: IC50 values (μM) for cells incubated in folate-free media
| Leukemia cell line . | KG-1 . | KG-1a . | L1210JF . | L1210 . | ||
|---|---|---|---|---|---|---|
| FR expression status . | FR-β (+) . | FR (−) . | FR-α (+) . | FR (−) . | ||
| Pre-exposure to ATRA . | − . | + . | − . | + . | − . | − . |
| Free DOX | 0.65 | 0.63 | 0.61 | 0.59 | 0.86 | 0.91 |
| f-L-DOX | 0.97 | 0.43 | 19 | 17 | 1.3 | 27 |
| Nontargeted L-DOX | 24 | 27 | 23 | 22 | 34 | 29 |
| Leukemia cell line . | KG-1 . | KG-1a . | L1210JF . | L1210 . | ||
|---|---|---|---|---|---|---|
| FR expression status . | FR-β (+) . | FR (−) . | FR-α (+) . | FR (−) . | ||
| Pre-exposure to ATRA . | − . | + . | − . | + . | − . | − . |
| Free DOX | 0.65 | 0.63 | 0.61 | 0.59 | 0.86 | 0.91 |
| f-L-DOX | 0.97 | 0.43 | 19 | 17 | 1.3 | 27 |
| Nontargeted L-DOX | 24 | 27 | 23 | 22 | 34 | 29 |
Assays were repeated twice. Within each assay, cells were treated in quadruplicate. The error within each group was less than 20%.