Cellular uptake of f-L-calcein by various cell lines determined by flow cytometry
| Cell line . | FR (+) . | FR (−) . | ||||||
|---|---|---|---|---|---|---|---|---|
| KG-1 . | L1210JF . | CHO-FR-β . | KG-1a . | L1210 . | CHO-K1 . | |||
| Pre-exposure to ATRA . | − . | + . | − . | − . | − . | + . | − . | − . |
| L-calcein | 9.3 ± 2.1 | 9.1 ± 1.3 | 5.7 ± 0.6 | 6.1 ± 1.3 | 5.4 ± 1.3 | 5.1 ± 0.3 | 4.7 ± 0.3 | 5.1 ± 2.3 |
| f-L-calcein | 61 ± 17 | 320 ± 78 | 78 ± 9 | 91 ± 12 | 6.1 ± 0.7 | 6.7 ± 0.9 | 7.1 ± 1.2 | 5.7 ± 1.5 |
| f-L-calcein + 1 mM folate | 11 ± 1.4 | 27 ± 2 | 8.3 ± 1.7 | 7.3 ± 0.2 | 5.1 ± 0.5 | 7.3 ± 1.4 | 6.4 ± 1.5 | 4.9 ± 0.7 |
| f-L-calcein + 1 μM folate | 63 ± 11 | ND | 81 ± 13 | ND | ND | ND | ND | ND |
| f-L-calcein + pretreated w/PI-PLC | 13 ± 4 | ND | ND | ND | ND | ND | ND | ND |
| Cell line . | FR (+) . | FR (−) . | ||||||
|---|---|---|---|---|---|---|---|---|
| KG-1 . | L1210JF . | CHO-FR-β . | KG-1a . | L1210 . | CHO-K1 . | |||
| Pre-exposure to ATRA . | − . | + . | − . | − . | − . | + . | − . | − . |
| L-calcein | 9.3 ± 2.1 | 9.1 ± 1.3 | 5.7 ± 0.6 | 6.1 ± 1.3 | 5.4 ± 1.3 | 5.1 ± 0.3 | 4.7 ± 0.3 | 5.1 ± 2.3 |
| f-L-calcein | 61 ± 17 | 320 ± 78 | 78 ± 9 | 91 ± 12 | 6.1 ± 0.7 | 6.7 ± 0.9 | 7.1 ± 1.2 | 5.7 ± 1.5 |
| f-L-calcein + 1 mM folate | 11 ± 1.4 | 27 ± 2 | 8.3 ± 1.7 | 7.3 ± 0.2 | 5.1 ± 0.5 | 7.3 ± 1.4 | 6.4 ± 1.5 | 4.9 ± 0.7 |
| f-L-calcein + 1 μM folate | 63 ± 11 | ND | 81 ± 13 | ND | ND | ND | ND | ND |
| f-L-calcein + pretreated w/PI-PLC | 13 ± 4 | ND | ND | ND | ND | ND | ND | ND |
Values shown represent the means of 3 measurements ± 1 SD.
ND indicates not determined.