FVIII activity and antigen values
| Factor VIII . | Database* . | Recombinant FVIII . | |||||
|---|---|---|---|---|---|---|---|
| Ac (U/dL) . | Ag (U/dL) . | Ratio (Ac/Ag) . | Ac (U/mL)† . | Ag (Ug/mL)‡ . | Ag (U/mL)1-153 . | Ratio (Ac/Ag) . | |
| WT | 250 | 53 | 159 | 1.57 | |||
| Ser558Phe | 21 | 175 | 0.12 | 23 | 115 | 345 | 0.07 |
| Val559Ala | 10 | 125 | 0.08 | 72.7 | 242 | 727 | 0.1 |
| Asp560Ala | 7 | – | – | 30 | 149 | 447 | 0.07 |
| Arg562Ala | 915 | 164 | 492 | 1.86 | |||
| Gln565Arg | 27/< 1 | 114/– | 0.24/– | 3.5 | 67 | 201 | 0.02 |
| Factor VIII . | Database* . | Recombinant FVIII . | |||||
|---|---|---|---|---|---|---|---|
| Ac (U/dL) . | Ag (U/dL) . | Ratio (Ac/Ag) . | Ac (U/mL)† . | Ag (Ug/mL)‡ . | Ag (U/mL)1-153 . | Ratio (Ac/Ag) . | |
| WT | 250 | 53 | 159 | 1.57 | |||
| Ser558Phe | 21 | 175 | 0.12 | 23 | 115 | 345 | 0.07 |
| Val559Ala | 10 | 125 | 0.08 | 72.7 | 242 | 727 | 0.1 |
| Asp560Ala | 7 | – | – | 30 | 149 | 447 | 0.07 |
| Arg562Ala | 915 | 164 | 492 | 1.86 | |||
| Gln565Arg | 27/< 1 | 114/– | 0.24/– | 3.5 | 67 | 201 | 0.02 |
Data were obtained from the hemophilia A mutation database (europium.csc.mrc.ac.uk/usr/WWW/WebPages/main.dir/main.htm). Ac and Ag refer to FVIII activity and antigen, respectively.
FVIII activity determined by a one-stage clotting assay as described in “Materials and methods.”
FVIII antigen determined using an ELISA as described in “Materials and methods.”
Units per milliliter were determined on the assumption that one unit of native FVIII is equivalent to 300 ng of protein.