Colony formation efficiency
BM infection . | IL-3 . | MCSF + IL-6 . |
---|---|---|
Neo | 143.3 ± 8.9 | 35.0 ± 2.9 |
NF-YA DN | 142.7 ± 9.1 | 52.7 ± 4.4 |
BM infection . | IL-3 . | MCSF + IL-6 . |
---|---|---|
Neo | 143.3 ± 8.9 | 35.0 ± 2.9 |
NF-YA DN | 142.7 ± 9.1 | 52.7 ± 4.4 |
Bone marrow (BM) cells were infected with Neo or dominant negative nuclear factor YA (NF-YA DN) retroviral particles, seeded in 1 mL methylcellulose supplemented with G418 (650 μg/mL), and treated with either interleukin 3 (IL-3; 10%) alone or macrophage colony-stimulating factor (MCSF) plus IL-6 (100 ng/mL). After 8 days G418-resistant colonies were counted. Data are the average of 3 independent experiments, with SE indicated. According to the Studentt test, the observed difference in the means of Neo–infected BM and NF-YA DN–infected BM treated with MCSF plus IL-6 is significant with more than 95% confidence.