Dual-FISH analysis of BM populations of an MDS case with combined del(5q) and trisomy 8
| Cell population . | FISH results, % of total cells . | |||
|---|---|---|---|---|
| Normal5-150 . | +8 alone . | 5q- alone . | 5q- and +8 . | |
| CD34+CD38−(HSCs) | 0 | 0 | 34 | 66 |
| CD34+CD38+(progenitors) | 0 | 0 | 80 | 20 |
| Granulocytic5-151 | 2 | 0 | 81 | 17 |
| Erythroid (normoblasts) | 12 | 0 | 82 | 6 |
| Cell population . | FISH results, % of total cells . | |||
|---|---|---|---|---|
| Normal5-150 . | +8 alone . | 5q- alone . | 5q- and +8 . | |
| CD34+CD38−(HSCs) | 0 | 0 | 34 | 66 |
| CD34+CD38+(progenitors) | 0 | 0 | 80 | 20 |
| Granulocytic5-151 | 2 | 0 | 81 | 17 |
| Erythroid (normoblasts) | 12 | 0 | 82 | 6 |
A patient with myelodysplastic syndrome (MDS) with both 5q deletion and trisomy 8 (patient no. 10) was investigated by dual fluorescence in situ hybridization (FISH) with DNA probes hybridizing to 5q31 and to chromosome 8. Note that regardless of the cell population investigated, the del(5q) was found in the majority but trisomy 8 only in a fraction of the cells. There were no cells with trisomy 8 alone (without concomitant 5q deletion). Hematopoietic stem cell (HSC) and progenitor populations were obtained by fluorescence activated cell sorting (FACS) purification whereas granulocyte and erythroid cell lineages were investigated by morphologic identification after May-Grünwald-Giemsa (MGG) staining.
Normal indicates cells with normal chromosome 5 and 8 status.
Includes all stages of granulocytic differentiation in the bone marrow (dysplastic and nondysplastic), except blasts.