Quantification of HTLV-I Tax and CD3 mRNA in whole blood, red cells, and plasma of seropositive asymptomatic carriers before and after leukocyte depletion by filtration
| Donor . | Whole blood before filtration HTLV-I copies/L (real-time PCR) . | Red cells after filtration HTLV-I copies/L (real-time PCR) . | Log10reduction before and after filtration HTLV-I (real-time PCR) . | Log10 reduction before and after filtration CD3 mRNA (real-time PCR) . | Red cells after filtration HTLV-I (nested PCR) . | Plasma prefiltration HTLV-I copies/L (real-time PCR) . | Plasma after filtration HTLV-I copies/L (real-time PCR) . | Residual WBC/μL* (flow cytometry) . |
|---|---|---|---|---|---|---|---|---|
| 1 | 1.61 × 109 | 4.44 × 104 | 4.56 | 3.96 | Not detected | 2.83 × 106 | 2.20 × 105 | 2.20 |
| 2 | 3.26 × 108 | 5.06 × 104 | 3.81 | 4.58 | Positive | 8.60 × 107 | 4.95 × 105 | 3.60 |
| 3 | 2.56 × 108 | Not detected | > 4.0 | 4.12 | Positive | 3.73 × 105 | 1.39 × 105 | < 1 |
| 4 | 2.34 × 107 | Not detected | > 3.2 | Not tested | Not detected | Not detected | Not detected | < 1 |
| 5 | 1.01 × 105 | Not detected | > 1 | 4.54 | Not detected | 3.30 × 104 | Not detected | 5.20 |
| Donor . | Whole blood before filtration HTLV-I copies/L (real-time PCR) . | Red cells after filtration HTLV-I copies/L (real-time PCR) . | Log10reduction before and after filtration HTLV-I (real-time PCR) . | Log10 reduction before and after filtration CD3 mRNA (real-time PCR) . | Red cells after filtration HTLV-I (nested PCR) . | Plasma prefiltration HTLV-I copies/L (real-time PCR) . | Plasma after filtration HTLV-I copies/L (real-time PCR) . | Residual WBC/μL* (flow cytometry) . |
|---|---|---|---|---|---|---|---|---|
| 1 | 1.61 × 109 | 4.44 × 104 | 4.56 | 3.96 | Not detected | 2.83 × 106 | 2.20 × 105 | 2.20 |
| 2 | 3.26 × 108 | 5.06 × 104 | 3.81 | 4.58 | Positive | 8.60 × 107 | 4.95 × 105 | 3.60 |
| 3 | 2.56 × 108 | Not detected | > 4.0 | 4.12 | Positive | 3.73 × 105 | 1.39 × 105 | < 1 |
| 4 | 2.34 × 107 | Not detected | > 3.2 | Not tested | Not detected | Not detected | Not detected | < 1 |
| 5 | 1.01 × 105 | Not detected | > 1 | 4.54 | Not detected | 3.30 × 104 | Not detected | 5.20 |
Sensitivity is 1 WBC/μL.