Reduction in HTLV Tax DNA and CD3 mRNA following leukocyte depletion of 16 whole blood and 8 platelet concentrates spiked with MT-2 cells
| Filter type . | Day of filtration . | HTLV-I log10 reduction (SD) . | CD3 mRNA log10 reduction (SD) . | Residual WBC/μL* flow cytometry (SD) . |
|---|---|---|---|---|
| Whole blood | ||||
| Pall WBF-2 | 0 | 3.55 (0.41) | 4.48 (0.59) | < 1 |
| Pall WBF-2 | 1 | 3.76 (0.69) | 4.60 (0.57) | < 1 |
| Baxter RZ2000 | 0 | 3.68 (0.68) | 4.87 (0.32) | < 1 |
| Baxter RZ2000 | 1 | 3.67 (0.83) | 4.88 (0.27) | < 1 |
| Mean of whole blood processes | 3.67 (0.60) | 4.71 (0.20) | < 1 | |
| Platelet | ||||
| Pall Autostop | 1 | 2.76 (0.55) | 3.78 (0.18) | 1.37 (0.46) |
| Baxter PLX-5 | 1 | 2.63 (0.68) | 4.15 (0.33) | < 1 |
| Mean of platelet processes | 2.70 (0.58) | 3.97 (0.31) | 1.11 (0.37) |
| Filter type . | Day of filtration . | HTLV-I log10 reduction (SD) . | CD3 mRNA log10 reduction (SD) . | Residual WBC/μL* flow cytometry (SD) . |
|---|---|---|---|---|
| Whole blood | ||||
| Pall WBF-2 | 0 | 3.55 (0.41) | 4.48 (0.59) | < 1 |
| Pall WBF-2 | 1 | 3.76 (0.69) | 4.60 (0.57) | < 1 |
| Baxter RZ2000 | 0 | 3.68 (0.68) | 4.87 (0.32) | < 1 |
| Baxter RZ2000 | 1 | 3.67 (0.83) | 4.88 (0.27) | < 1 |
| Mean of whole blood processes | 3.67 (0.60) | 4.71 (0.20) | < 1 | |
| Platelet | ||||
| Pall Autostop | 1 | 2.76 (0.55) | 3.78 (0.18) | 1.37 (0.46) |
| Baxter PLX-5 | 1 | 2.63 (0.68) | 4.15 (0.33) | < 1 |
| Mean of platelet processes | 2.70 (0.58) | 3.97 (0.31) | 1.11 (0.37) |
Results are mean (SD) of 4 replicates.
Sensitivity is 1 WBC/μL.