Antibodies used in this study with used staining conditions
| Antigen . | Antibody . | Titer . | Treatment . |
|---|---|---|---|
| Procaspase 3 | CPP32 | 1:500 | Citrate, sABC, CARD |
| Procaspase 3 | Clone 19 | 1:10 | Citrate, sABC, CARD |
| Active caspase 3 | C92-605 | 1:1000 | Citrate, sABC, CARD |
| PARP-1 | C2-10 | 1:500 | Citrate, sABC, CARD |
| PARP-1/p89 | — | 1:1000 | Citrate, sABC, CARD |
| P53 | DO-7 | 1:500 | Citrate, o/n, sABC |
| P21 | Waf-1 | 1:50 | Citrate, o/n, sABC |
| Granzyme B | GrB7 | 1:500 | Citrate, sABC |
| Antigen . | Antibody . | Titer . | Treatment . |
|---|---|---|---|
| Procaspase 3 | CPP32 | 1:500 | Citrate, sABC, CARD |
| Procaspase 3 | Clone 19 | 1:10 | Citrate, sABC, CARD |
| Active caspase 3 | C92-605 | 1:1000 | Citrate, sABC, CARD |
| PARP-1 | C2-10 | 1:500 | Citrate, sABC, CARD |
| PARP-1/p89 | — | 1:1000 | Citrate, sABC, CARD |
| P53 | DO-7 | 1:500 | Citrate, o/n, sABC |
| P21 | Waf-1 | 1:50 | Citrate, o/n, sABC |
| Granzyme B | GrB7 | 1:500 | Citrate, sABC |
Citrate, antigen retrieval with citrate buffer (10 mM, pH 6.0, at 700 W for 10 minutes); o/n, overnight incubation of the primary antibody; sABC, standard 3-step ABC method; CARD, catalyzed reported deposition enhancement of the staining signal.