Checkerboard analysis of the SCF-induced cell migration of BAF3/KITWT cells
| Chemotaxis of BAF3/KITWT cells (% cells in lower chamber/whole cells) . | ||||||
|---|---|---|---|---|---|---|
| SCF concentration (ng/mL) in lower chamber . | SCF concentration (ng/mL) in upper chamber . | |||||
| 0 . | 1 . | 3 . | 10 . | 30 . | 100 . | |
| 0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.0 | 0.1 ± 0.0 |
| 1 | 0.2 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 3 | 1.4 ± 0.1 | 1.0 ± 0.1 | 0.7 ± 0.0 | 0.3 ± 0.0 | 0.2 ± 0.0 | 0.2 ± 0.0 |
| 10 | 17.9 ± 1.3 | 14.8 ± 0.7 | 13.3 ± 0.9 | 5.4 ± 0.3 | 3.6 ± 0.4 | 1.2 ± 0.1 |
| 30 | 37.2 ± 3.6 | 32.2 ± 1.9 | 22.7 ± 2.5 | 14.0 ± 1.1 | 7.6 ± 0.5 | 1.2 ± 0.1 |
| 100 | 10.5 ± 0.9 | 8.5 ± 0.8 | 8.5 ± 0.7 | 8.5 ± 0.4 | 3.2 ± 0.3 | 0.5 ± 0.0 |
| Chemotaxis of BAF3/KITWT cells (% cells in lower chamber/whole cells) . | ||||||
|---|---|---|---|---|---|---|
| SCF concentration (ng/mL) in lower chamber . | SCF concentration (ng/mL) in upper chamber . | |||||
| 0 . | 1 . | 3 . | 10 . | 30 . | 100 . | |
| 0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.0 | 0.1 ± 0.0 |
| 1 | 0.2 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 3 | 1.4 ± 0.1 | 1.0 ± 0.1 | 0.7 ± 0.0 | 0.3 ± 0.0 | 0.2 ± 0.0 | 0.2 ± 0.0 |
| 10 | 17.9 ± 1.3 | 14.8 ± 0.7 | 13.3 ± 0.9 | 5.4 ± 0.3 | 3.6 ± 0.4 | 1.2 ± 0.1 |
| 30 | 37.2 ± 3.6 | 32.2 ± 1.9 | 22.7 ± 2.5 | 14.0 ± 1.1 | 7.6 ± 0.5 | 1.2 ± 0.1 |
| 100 | 10.5 ± 0.9 | 8.5 ± 0.8 | 8.5 ± 0.7 | 8.5 ± 0.4 | 3.2 ± 0.3 | 0.5 ± 0.0 |
Data are shown as the percentages of cells in the lower chamber to whole applied cells. Results presented the mean ± SD of triplicate cultures. The experiments were repeated at least 3 times with similar results.