Percentages of NOD/SCID mice chimeric for human CD45+ cells after transplantation with human FL or FBM CD34+ cells isolated in G0, G1, or S/G2 + M phases of cell cycle
| Cell cycle status of graft . | Chimerism . | |||||
|---|---|---|---|---|---|---|
| Bone marrow . | Spleen . | Peripheral blood . | ||||
| FL . | FBM . | FL . | FBM . | FL . | FBM . | |
| G0 | 55 (n = 11) | 60 (n = 10) | 67 (n = 9) | 13 (n = 8) | 67 (n = 9) | 29 (n = 7) |
| (82)* | (90) | (89) | (75) | (89) | (100) | |
| G1 | 60 (n = 10) | 22 (n = 9) | 50 (n = 8) | 0 (n = 6) | 57 (n = 7) | 29 (n = 7) |
| (90) | (45) | (75) | (67) | (100) | (86) | |
| S/G2 + M | 60 (n = 5) | 29 (n = 7) | 75 (n = 4) | 20 (n = 5) | 50 (n = 4) | 50 (n = 6) |
| (80) | (86) | (75) | (80) | (75) | (100) | |
| Cell cycle status of graft . | Chimerism . | |||||
|---|---|---|---|---|---|---|
| Bone marrow . | Spleen . | Peripheral blood . | ||||
| FL . | FBM . | FL . | FBM . | FL . | FBM . | |
| G0 | 55 (n = 11) | 60 (n = 10) | 67 (n = 9) | 13 (n = 8) | 67 (n = 9) | 29 (n = 7) |
| (82)* | (90) | (89) | (75) | (89) | (100) | |
| G1 | 60 (n = 10) | 22 (n = 9) | 50 (n = 8) | 0 (n = 6) | 57 (n = 7) | 29 (n = 7) |
| (90) | (45) | (75) | (67) | (100) | (86) | |
| S/G2 + M | 60 (n = 5) | 29 (n = 7) | 75 (n = 4) | 20 (n = 5) | 50 (n = 4) | 50 (n = 6) |
| (80) | (86) | (75) | (80) | (75) | (100) | |
Except for n values, all numbers are percentages.
Data reflect percentages of chimeric animals (1% or more CD45+ cells) determined after analysis of each tissue (BM, spleen, or peripheral blood). Number of animals available for analysis is shown as n for every group.
Denotes percentage of chimeric animals at the 0.1% or higher CD45+ level in every group.