CFU content and LTC-IC frequency of different fractions of FBM, FL, and FB cells fractionated based on their cell cycle position
| Tissue source . | Fraction . | Size of each fraction* . | Total HPCs† . | LTC-ICs . |
|---|---|---|---|---|
| Fetal BM | G0CD34+ | 25.5 | 180 ± 41 (46)‡ | 5 |
| G1CD34+ | 59.5 | 250 ± 47 (150) | 4 | |
| S/G2 + M CD34+ | 15.0 | 240 (36) | 2 | |
| FL | G0CD34+ | 24.1 | 75 ± 15 (18) | 9 |
| G1CD34+ | 66.7 | 88 ± 23 (59) | 8 | |
| S/G2 + M CD34+ | 9.2 | 110 (11) | 7 | |
| FB | G0CD34+Lin− | 15.5 | 130 ± 26 (19) | ND |
| G1CD34+Lin− | 67.3 | 140 ± 15 (91) | ND |
| Tissue source . | Fraction . | Size of each fraction* . | Total HPCs† . | LTC-ICs . |
|---|---|---|---|---|
| Fetal BM | G0CD34+ | 25.5 | 180 ± 41 (46)‡ | 5 |
| G1CD34+ | 59.5 | 250 ± 47 (150) | 4 | |
| S/G2 + M CD34+ | 15.0 | 240 (36) | 2 | |
| FL | G0CD34+ | 24.1 | 75 ± 15 (18) | 9 |
| G1CD34+ | 66.7 | 88 ± 23 (59) | 8 | |
| S/G2 + M CD34+ | 9.2 | 110 (11) | 7 | |
| FB | G0CD34+Lin− | 15.5 | 130 ± 26 (19) | ND |
| G1CD34+Lin− | 67.3 | 140 ± 15 (91) | ND |
Sizes of G0, G1, and S/G2 + M fractions reported here are derived from the analysis of the FBM and FL samples shown in Figure 1. Similar results were obtained from the analysis of other samples used in these studies. Values obtained from the analysis of FB G0CD34+Lin− and G1CD34+Lin− fractions from one representative sample are also shown. In this particular sample, cells in S/G2 + M comprised 17.2% of CD34+Lin− cells analyzed.
Total number of clonogenic HPCs and frequency of LTC-ICs per 1 × 103 evaluated cells. Data are reported as mean ± SD of 2 to 4 samples for G0 and G1fractions of FBM and FL cells (assayed in duplicate) and 5 samples (assayed in triplicates) for FB. Only one sample each of FBM and FL CD34+ cells in S/G2 + M from the same fetus was assayed. LTC-IC data were collected from one set of FBM and FL tissues collected from the same fetus. No statistically significant differences were detected between groups.
Numbers in parentheses reflect the relative distribution of progenitors among different phases of the cell cycle. These values were obtained by multiplying the number of progenitor cells for every fraction by the percentage that fraction represents of total cells analyzed, as shown in Figure 1, panels C and D.
ND indicates not determined.