Table 3.

Phenotype of plasmacytoma cells cocultured with CL7.1/neo or CL7.1/mCD40L fibroblasts

Cell type and
coculture
Time of coculture, h% of positive cells3-150
CD80CD86I-Ad/I-Ed
MPC-11 Fresh cells 6 ± 1.3 26 ± 20 59 ± 19 
 CL7.1/neo 24 9 ± 4.2 27 ± 17 83 ± 9.3 
 48 4 ± 2.7 33 ± 16.2 65 ± 28.5 
 72 2 ± 1.9 29 ± 7.2 79 ± 4.5 
 CL7.1/mCD40L 24 16 ± 9 22 ± 18 70 ± 24.6 
 48 7 ± 3.4 44 ± 26 85 ± 2.9 
 72 2 ± 1 21 ± 6.1 82 ± 8.5 
S107 Fresh cells 35 ± 28 12 ± 6 41 ± 20 
 CL7.1/neo 24 35 ± 34 24 ± 11 78 ± 6 
 48 21 ± 7.3 28 ± 24 78 ± 17.9 
 72 19 ± 8.1 29 ± 9.7 73 ± 24.1 
 CL7.1/mCD40L 24 31 ± 20 18 ± 18 72 ± 17 
 48 25 ± 11 22 ± 19 72 ± 12 
 72 22 ± 12 28 ± 14 69 ± 25  
B splenocytes Fresh cells 33 ± 14 56.8 ± 25 98 ± 1.3 
 CL7.1/neo 24 13 ± 3 39 ± 7 92 ± 4 
 48 17.7 ± 5 43.1 ± 32.6 80.4 ± 14 
 72 21.4 ± 3 67.5 ± 24.7 84.6 ± 6.5 
 CL7.1/mCD40L 24 25.7 ± 4.7 89.6 ± 7.9 83.5 ± 19 
 48 74.1 ± 10.13-151 78.5 ± 2 81 ± 3 
 72 62 ± 17 88.1 ± 11 79.5 ± 5 
Cell type and
coculture
Time of coculture, h% of positive cells3-150
CD80CD86I-Ad/I-Ed
MPC-11 Fresh cells 6 ± 1.3 26 ± 20 59 ± 19 
 CL7.1/neo 24 9 ± 4.2 27 ± 17 83 ± 9.3 
 48 4 ± 2.7 33 ± 16.2 65 ± 28.5 
 72 2 ± 1.9 29 ± 7.2 79 ± 4.5 
 CL7.1/mCD40L 24 16 ± 9 22 ± 18 70 ± 24.6 
 48 7 ± 3.4 44 ± 26 85 ± 2.9 
 72 2 ± 1 21 ± 6.1 82 ± 8.5 
S107 Fresh cells 35 ± 28 12 ± 6 41 ± 20 
 CL7.1/neo 24 35 ± 34 24 ± 11 78 ± 6 
 48 21 ± 7.3 28 ± 24 78 ± 17.9 
 72 19 ± 8.1 29 ± 9.7 73 ± 24.1 
 CL7.1/mCD40L 24 31 ± 20 18 ± 18 72 ± 17 
 48 25 ± 11 22 ± 19 72 ± 12 
 72 22 ± 12 28 ± 14 69 ± 25  
B splenocytes Fresh cells 33 ± 14 56.8 ± 25 98 ± 1.3 
 CL7.1/neo 24 13 ± 3 39 ± 7 92 ± 4 
 48 17.7 ± 5 43.1 ± 32.6 80.4 ± 14 
 72 21.4 ± 3 67.5 ± 24.7 84.6 ± 6.5 
 CL7.1/mCD40L 24 25.7 ± 4.7 89.6 ± 7.9 83.5 ± 19 
 48 74.1 ± 10.13-151 78.5 ± 2 81 ± 3 
 72 62 ± 17 88.1 ± 11 79.5 ± 5 

See Table 2 for abbreviations.

F3-150

Data are represented as a percentage of positive cells ± SD of 3 independent experiments.

F3-151

Results statistically significant; P = .02.

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