Table 2.

Numbers and erythroid differentiation of clones produced by different subsets of CB34+ CB cells transduced with MIG or p210 and plated in single-cell serum-free liquid cultures

Subsets of CB cellsEpoTotal clones (%)No. of erythroid clones/no. analyzed (%)*
MIGp210MIGp210
CD34+CD45RACD71(stem cell candidates) 69/192 (36) 103/192 (54) 8/14 (57) 20/21 (95) 
− 24/96 (25) 41/96 (43) 8/17 (47) 12/16 (75) 
CD34+CD45RA+CD71(granulopoietic) 62/192 (32) 84/192 (44) 7/23 (30) 21/24 (88) 
− 17/96 (18) 22/96 (23) 1/10 (10) 8/14 (57) 
CD34+CD45RACD71+(erythroid) 102/192 (53) 139/192 (72) 20/22 (91) 22/22 (100) 
− 8/96 (8) 2/96 (2) 8/8 (100) 3/3 (100) 
Subsets of CB cellsEpoTotal clones (%)No. of erythroid clones/no. analyzed (%)*
MIGp210MIGp210
CD34+CD45RACD71(stem cell candidates) 69/192 (36) 103/192 (54) 8/14 (57) 20/21 (95) 
− 24/96 (25) 41/96 (43) 8/17 (47) 12/16 (75) 
CD34+CD45RA+CD71(granulopoietic) 62/192 (32) 84/192 (44) 7/23 (30) 21/24 (88) 
− 17/96 (18) 22/96 (23) 1/10 (10) 8/14 (57) 
CD34+CD45RACD71+(erythroid) 102/192 (53) 139/192 (72) 20/22 (91) 22/22 (100) 
− 8/96 (8) 2/96 (2) 8/8 (100) 3/3 (100) 
*

Data shown are the number of clones detected/number of wells analyzed (expressed as a % value in parentheses) for both total clones (≥ 32 cells per well, assessed by direct visualization in situ after 7 days of culture after transduction) and for erythroid clones. The frequency of erythroid clones was determined by morphologic analysis of Wright-Giemsa–stained cytospin preparations made individually on randomly selected 7-day-old clones of ≥ 32 cells/well. Data are from 2 experiments with epo (+) and 1 experiment without epo (−). All wells contained FL, SF, IL-3, IL-6, and G-CSF with or without (±) epo as indicated.

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