Absolute and relative frequencies of myelomonocytes, DCs, NK cells, and T cells generated from CD34hi early thymic precursors and from their in vitro–derived progenies
| Progenitors . | DCs* (%) . | NK cells* (%) . | TCRαβ+ T cells† (%) . | Myelomonocytes‡ (%) . |
|---|---|---|---|---|
| CD34hiCD33lo | 650 (44) | 780 (53) | 14.3 (43) | 33.6 (15) |
| CD34loCD44hi | 210 (35) | 390 (65) | < 0.05 (4) | 10 (17) |
| CD34loCD44lo | < 1 (1) | 104 (95) | 9 (60) | < 0.05 (< 1) |
| Progenitors . | DCs* (%) . | NK cells* (%) . | TCRαβ+ T cells† (%) . | Myelomonocytes‡ (%) . |
|---|---|---|---|---|
| CD34hiCD33lo | 650 (44) | 780 (53) | 14.3 (43) | 33.6 (15) |
| CD34loCD44hi | 210 (35) | 390 (65) | < 0.05 (4) | 10 (17) |
| CD34loCD44lo | < 1 (1) | 104 (95) | 9 (60) | < 0.05 (< 1) |
Absolute numbers (× 10−3) and percentages (in parentheses) of DCs, NK cells, T cells, and myelomonocytes generated either from freshly isolated CD34hiCD33lothymic progenitors or from their CD34loCD44hiand CD34loCD44lo progenies derived by day 3.5 in multicytokine-supported cultures.
Generation of NK cells and DCs per 105 input cell progenitors was determined in IL-2–supplemented multicytokine-supported cultures by day 9.
Absolute numbers and percentages of TCRαβ+T cells recovered per fetal thymic lobe reconstituted with 2 × 104 progenitors were determined after 25 days in hu/moFTOC.
Generation of monocytes (CD14+CD1a−) per 105 input progenitors was determined by day 7 in cultures supplemented with M-CSF, IL-7, and SCF.
DCs indicates dendritic cells; NK, natural killer; TCR, T-cell receptor; hu/moFTOC, hybrid human/mouse fetal thymic organ culture; M-CSF, macrophage–colony-stimulating factor; IL-7, interleukin 7; SCF, stem cell factor.