SDF-1 expression in HUVECs
| Test sample . | Total protein (ng ± SD) . | SDF-1 (ng ± SD) . |
|---|---|---|
| HUVEC membrane | 25.0 × 106 ± 77.2 | 10.36 ± 0.2 |
| HUVEC cell lysate | 38.0 × 104 ± 20.7 | 1.58 ± 0.1 |
| Daudi cell membrane | 18.5 × 106 ± 23.0 | ND |
| T-lymphocyte cell lysate | 7.5 × 106 ± 37.6 | ND |
| Test sample . | Total protein (ng ± SD) . | SDF-1 (ng ± SD) . |
|---|---|---|
| HUVEC membrane | 25.0 × 106 ± 77.2 | 10.36 ± 0.2 |
| HUVEC cell lysate | 38.0 × 104 ± 20.7 | 1.58 ± 0.1 |
| Daudi cell membrane | 18.5 × 106 ± 23.0 | ND |
| T-lymphocyte cell lysate | 7.5 × 106 ± 37.6 | ND |
Cell membrane preparations derived from approximately 107 cells and cell lysates derived from approximately 2 × 106 cells were tested for SDF-1 content by specific ELISA. Data represent the means of triplicate determinations (± SD).
ND indicates not detected.