Relative IL-3R surface expression in HD cell lines
| Cell line . | Origin . | EBV status . | IL-3R–associated surface fluorescence . |
|---|---|---|---|
| DAUDI | B | + | 0.3 |
| RAJI | B | + | 0.6 |
| JJAN | T | − | 0.0 |
| SUPT1 | T | − | 0.0 |
| KM-H2 | HD | − | 0.0 |
| HO | HD | − | 0.0 |
| L591 | HD | + | 6.5 |
| L1236 | HD | − | 10.5 |
| HDLM-2 | HD | − | 60.6 |
| L428 | HD | − | 100.0 |
| Cell line . | Origin . | EBV status . | IL-3R–associated surface fluorescence . |
|---|---|---|---|
| DAUDI | B | + | 0.3 |
| RAJI | B | + | 0.6 |
| JJAN | T | − | 0.0 |
| SUPT1 | T | − | 0.0 |
| KM-H2 | HD | − | 0.0 |
| HO | HD | − | 0.0 |
| L591 | HD | + | 6.5 |
| L1236 | HD | − | 10.5 |
| HDLM-2 | HD | − | 60.6 |
| L428 | HD | − | 100.0 |
HD cell lines (KM-H2, HO, L591, L1236, HDLM-2, L428) were labeled with a murine monoclonal antibody directed against human IL-3R (7G3, Cambridge Bioscience, Cambridge, United Kingdom) and cell-bound IgG visualized with phycoerythrin (PE)-conjugated antimouse IgG (Dako, Glostrup, Denmark). Relative surface fluorescence was determined by flow cytometry (FACSCalibur, Becton Dickinson, Franklin Lakes, NJ). Human B (DAUDI, RAJI) and T (JJAN, SUPT1) lymphocyte cell lines were used as controls. Not represented among HD cell lines are HD tumors containing Epstein-Barr virus (EBV)–positive HRS cells, which account for approximately half of all HD cases.