Table 2. Effects of EphB4 on... | American Society of Hematology

Table 2.

Effects of EphB4 on colony-forming ability of CD34⁺ cells

Sample no.	CFU-GM		BFU/CFU-E		CFU-GEMM		Total CFCs		CFU-Meg
Sample no.	Vector	EphB4	Vector	EphB4	Vector	EphB4	Vector	EphB4	Vector	EphB4
1	82	111	29	43	5	7	114	161	38.0	48.0
2	102	128	25	32	5	4	132	164	—	—
3	13	30	14	12	3	3	30	45	9.6	14.4
4	96	109	23	47	9	17	128	171	18.4	32.4
5	21	27	12	17	10	11	43	55	12.8	13.2
Mean	63	81	20.6	30	6.4	8.4	89.4	119.2	19.7	27.0
P	.006		.047		.137		.007		.045

Sample no.	CFU-GM		BFU/CFU-E		CFU-GEMM		Total CFCs		CFU-Meg
Sample no.	Vector	EphB4	Vector	EphB4	Vector	EphB4	Vector	EphB4	Vector	EphB4
1	82	111	29	43	5	7	114	161	38.0	48.0
2	102	128	25	32	5	4	132	164	—	—
3	13	30	14	12	3	3	30	45	9.6	14.4
4	96	109	23	47	9	17	128	171	18.4	32.4
5	21	27	12	17	10	11	43	55	12.8	13.2
Mean	63	81	20.6	30	6.4	8.4	89.4	119.2	19.7	27.0
P	.006		.047		.137		.007		.045

Three hundred transduced CD34⁺/GFP⁺ cells were plated in triplicate 24-well plates with 1% methylcellulose containing SCF, IL-3, IL-6, erythropoietin, GM-CSF, and G-CSF for total CFCs. Colonies were counted from each of the plates, and averages were determined for each individual. CFU-Meg colonies were determined separately using collagen-based slide chambers in the presence of TPO, IL-3, and IL-6, at a density of 2500 cells per chamber. All colony numbers were quantified using an inverted-phase light microscope.P values were calculated using Wilcoxon matched-pairs signed-rank test.

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