Table 1. Effect of EphB4 on primary... | American Society of Hematology

Table 1.

Effect of EphB4 on primary human CD34⁺ cells cultured with SCF, TPO, and FL

	CD41		GlycophrinA		CD14		CD33
	Vector	EphB4	Vector	EphB4	Vector	EphB4	Vector	EphB4
Sample no.
1	4.1	13.8	3.8	11.1	—	—	—	—
2	2.9	8.4	2.0	10.5	27.2	40.3	65.4	67.4
3	6.8	15.2	2.8	4.6	41.0	33.9	97.4	97.8
4	30.2	59.8	18.8	31.7	54.5	44.7	97.2	98.9
5	24.4	35.4	10.2	25.2	43.5	44.6	89.5	98.1
Mean	13.7	26.5	7.5	16.6	41.6	40.9	87.4	90.6
P	.020		.008		.131		.092

	CD41		GlycophrinA		CD14		CD33
	Vector	EphB4	Vector	EphB4	Vector	EphB4	Vector	EphB4
Sample no.
1	4.1	13.8	3.8	11.1	—	—	—	—
2	2.9	8.4	2.0	10.5	27.2	40.3	65.4	67.4
3	6.8	15.2	2.8	4.6	41.0	33.9	97.4	97.8
4	30.2	59.8	18.8	31.7	54.5	44.7	97.2	98.9
5	24.4	35.4	10.2	25.2	43.5	44.6	89.5	98.1
Mean	13.7	26.5	7.5	16.6	41.6	40.9	87.4	90.6
P	.020		.008		.131		.092

CD34⁺ cells were transduced with MSCV-GFP (vector) or MSCV-EphB4 (EphB4). GFP⁺ cells were sorted and cultured in the presence of SCF (50 ng/mL), TPO (25 ng/mL), and flt3 ligand (FL) (50 ng/mL) for 7 days. P values were calculated using Wilcoxon matched-pairs signed-rank test.

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