Table 1. Results of CRU assays of cells... | American Society of Hematology

Table 1.

Results of CRU assays of cells from secondary cultures of lin⁻ CB cells

Experiment no.	SF/TPO		SF/TPO + SDF-1		SF/TPO + TGF-β₁		SF/TPO + SDF-1 + TGF-β₁		5 GFs^*
Experiment no.	Cells per mouse, × 10⁶	L⁺ + M⁺	Cells per mouse, × 10⁶	L⁺ + M⁺	Cells per mouse, × 10⁶	L⁺ + M⁺	Cells per mouse, × 10⁶	L⁺ + M⁺	Cells per mouse, × 10⁶	L⁺ + M⁺
1	1.3	5/6	1.3	6/6	1.3	6/6	1.3	5/6	1.3	6/6
	0.3	3/6	0.3	4/6	0.3	4/6	0.3	4/6	0.3	2/6
2	1.0	5/6	1.0	6/6	1.0	5/6	1.0	5/6	1.0	5/6
	0.4	1/6	0.4	3/6	0.4	1/5	0.4	1/6	0.4	3/6
3	2.0	6/6	2.0	5/5	2.0	6/6	2.0	6/6	2.0	6/6
	0.4	5/6	0.4	3/3	0.4	2/4	0.4	2/6	0.4	2/6
CRUs per 10⁶ input cells in second cultures (range defined by SEM)	2.0 (1.6-2.6)		4.6 (3.4-6.2)		2.3 (1.8-3.0)		1.7 (1.3-2.2)		2.0 (1.5-2.5)

Experiment no.	SF/TPO		SF/TPO + SDF-1		SF/TPO + TGF-β₁		SF/TPO + SDF-1 + TGF-β₁		5 GFs^*
Experiment no.	Cells per mouse, × 10⁶	L⁺ + M⁺	Cells per mouse, × 10⁶	L⁺ + M⁺	Cells per mouse, × 10⁶	L⁺ + M⁺	Cells per mouse, × 10⁶	L⁺ + M⁺	Cells per mouse, × 10⁶	L⁺ + M⁺
1	1.3	5/6	1.3	6/6	1.3	6/6	1.3	5/6	1.3	6/6
	0.3	3/6	0.3	4/6	0.3	4/6	0.3	4/6	0.3	2/6
2	1.0	5/6	1.0	6/6	1.0	5/6	1.0	5/6	1.0	5/6
	0.4	1/6	0.4	3/6	0.4	1/5	0.4	1/6	0.4	3/6
3	2.0	6/6	2.0	5/5	2.0	6/6	2.0	6/6	2.0	6/6
	0.4	5/6	0.4	3/3	0.4	2/4	0.4	2/6	0.4	2/6
CRUs per 10⁶ input cells in second cultures (range defined by SEM)	2.0 (1.6-2.6)		4.6 (3.4-6.2)		2.3 (1.8-3.0)		1.7 (1.3-2.2)		2.0 (1.5-2.5)

In each of the 3 experiments performed, more than 90% of all the cells from each culture were injected at the doses shown into a minimum of 8 irradiated NOD/SCID mice. L⁺ indicates lymphoid engraftment with human cells (CD34⁻CD19/20⁺); M⁺, myeloid engraftment with human cells (CD45/71⁺CD15/66b⁺)

The 5 GFs were Flt3 ligand, SF, interleukin-3, interleukin-6, and granulocyte colony-stimulating factor, as described in “Materials and methods.”

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