Changes in lymph node cell type and number after treatment with rGM-CSF and rGM-CSF/rIL-4
. | Cells (%) . | Total cells (× 106) . | ||||
---|---|---|---|---|---|---|
Control . | GM . | GM/IL-4 . | Control . | GM . | GM/IL-4 . | |
B220 | 14.3 ± 1.9 | 20.8 ± 1.9 | 16.0 ± 3.2 | 1.0 ± 0.7 | 1.8 ± 0.6 | 2.2 ± 0.8 |
Thy1.2 | 78.5 ± 3.6 | 69.3 ± 1.0 | 71.2 ± 4.0 | 5.4 ± 3.3 | 6.8 ± 1.9 | 10.0 ± 4.3 |
NK1.1 | 0.5 ± 0.0 | 0.7 ± 0.0 | 1.1 ± 0.2 | 0.03 ± 0.03 | 0.5 ± 0.4 | 0.1 ± 0.1 |
CD11b+ | 5.1 ± 0.6 | 7.9 ± 0.8 | 5.6 ± 0.4 | 0.3 ± 0.2 | 0.7 ± 0.2 | 0.8 ± 0.8 |
Total | 100.5 ± 3.6 | 97.4 ± 3.8 | 95.0 ± 4.3 | 6.8 ± 4.4 | 10.0 ± 3.0 | 13.2 ± 5.7 |
. | Cells (%) . | Total cells (× 106) . | ||||
---|---|---|---|---|---|---|
Control . | GM . | GM/IL-4 . | Control . | GM . | GM/IL-4 . | |
B220 | 14.3 ± 1.9 | 20.8 ± 1.9 | 16.0 ± 3.2 | 1.0 ± 0.7 | 1.8 ± 0.6 | 2.2 ± 0.8 |
Thy1.2 | 78.5 ± 3.6 | 69.3 ± 1.0 | 71.2 ± 4.0 | 5.4 ± 3.3 | 6.8 ± 1.9 | 10.0 ± 4.3 |
NK1.1 | 0.5 ± 0.0 | 0.7 ± 0.0 | 1.1 ± 0.2 | 0.03 ± 0.03 | 0.5 ± 0.4 | 0.1 ± 0.1 |
CD11b+ | 5.1 ± 0.6 | 7.9 ± 0.8 | 5.6 ± 0.4 | 0.3 ± 0.2 | 0.7 ± 0.2 | 0.8 ± 0.8 |
Total | 100.5 ± 3.6 | 97.4 ± 3.8 | 95.0 ± 4.3 | 6.8 ± 4.4 | 10.0 ± 3.0 | 13.2 ± 5.7 |
Axillary and inguinal lymph nodes were recovered from mice and were processed in a manner identical to that used for spleen cells. Data represent the mean ± SD (n = 3 experiments).