Light chain Jk gene usage and Jk sequences of 6-19–like IgG3 anti-IgG2a RF mAb derived from 6-19 heavy-chain transgenic mice
| Fusion . | mAb3-150 . | Jk . | Jk sequence† . |
|---|---|---|---|
| 6-19 | Jk2′ | YTFGSGTKLEIK | |
| I | A6C | Jk2′ | ------------ |
| G1H | Jk2 | ----G------- | |
| S1B | Jk4 | F----------- | |
| C12A | Jk1 | P---G------- | |
| II | P11C | Jk2′ | ------------ |
| E21A | Jk1 | R---G------- | |
| Q4C | Jk1 | W---G------- | |
| R11A | Jk4 | ●---T-------- | |
| L10H | Jk4 | F---T------- | |
| D6G | Jk5 | L---A-----L- | |
| III | Y12C | Jk2′ | ------------ |
| Z5C | Jk1 | W---G------- |
| Fusion . | mAb3-150 . | Jk . | Jk sequence† . |
|---|---|---|---|
| 6-19 | Jk2′ | YTFGSGTKLEIK | |
| I | A6C | Jk2′ | ------------ |
| G1H | Jk2 | ----G------- | |
| S1B | Jk4 | F----------- | |
| C12A | Jk1 | P---G------- | |
| II | P11C | Jk2′ | ------------ |
| E21A | Jk1 | R---G------- | |
| Q4C | Jk1 | W---G------- | |
| R11A | Jk4 | ●---T-------- | |
| L10H | Jk4 | F---T------- | |
| D6G | Jk5 | L---A-----L- | |
| III | Y12C | Jk2′ | ------------ |
| Z5C | Jk1 | W---G------- |
The 6-19 monoclonal antibody (mAb) is derived from an MRL-Faslpr mouse.17 The 6-19–like IgG3 anti-IgG2a RF mAbs bearing the 6-19 idiotype, resulting from the combination of the transgenic 6-19 heavy chains with endogenous light chains, were established from 3 independent fusions by using spleen cells from 6-19 heavy-chain transgenic mice, and all of them utilize the Vk1c gene segment identical to that of the 6-19 mAb. †Dashes refer to amino acid residue identical to the 6-19 Jk sequence, and a gap is indicated by a dot.