Enrichment of transfused reticulocytes from recipient mice
| Source of blood . | Type of sample . | Reticulocytes, % . | Biotinylated, % . |
|---|---|---|---|
| BALB/c recipient-reticulocyte-rich cells transfused (6 h) | Blood smear | 4.63 | ND |
| Column retentate | 29.5 | 76.8 | |
| Column eluate | 1.8 | 6.5 | |
| BALB/c recipient-normocyte-rich cells transfused (6 h) | Blood smear | 1.13 | ND |
| Column retentate | 0.95 | 70.0 | |
| Column eluate | 1.6 | 16.0 |
| Source of blood . | Type of sample . | Reticulocytes, % . | Biotinylated, % . |
|---|---|---|---|
| BALB/c recipient-reticulocyte-rich cells transfused (6 h) | Blood smear | 4.63 | ND |
| Column retentate | 29.5 | 76.8 | |
| Column eluate | 1.8 | 6.5 | |
| BALB/c recipient-normocyte-rich cells transfused (6 h) | Blood smear | 1.13 | ND |
| Column retentate | 0.95 | 70.0 | |
| Column eluate | 1.6 | 16.0 |
Reticulocyte-rich blood from DKO or WT mice was biotinylated and transfused into 5-day P yoelii–infected BALB/c mice, and blood was sampled at 6 hours after transfusion. Reticulocytes were enumerated in cells positively selected by a magnetic column.
ND indicates not done.