Table 1. Effect of photodynamic cell... | American Society of Hematology

Table 1.

Effect of photodynamic cell purging process on antigen-specific interferon-γ secretion measured by enzyme-linked immunospot assay

Groups	Responder only	Against BALB/c		Against C3H/HeJ
Groups	SPOTS	SPOTS	SI	SPOTS	SI
Naı̈ve C57BL/6	22 ± 11	96 ± 11	4.4 ± 0.5	63 ± 7	2.9 ± 0.3
Untreated primed cells	64 ± 64	2672 ± 68	36.6 ± 1.0	181 ± 103	2.5 ± 1.4
PDP-treated primed cells	73 ± 12	650 ± 42^*,^†	10.2 ± 0.7^*,^†	144 ± 11	2.3 ± 0.2

Groups	Responder only	Against BALB/c		Against C3H/HeJ
Groups	SPOTS	SPOTS	SI	SPOTS	SI
Naı̈ve C57BL/6	22 ± 11	96 ± 11	4.4 ± 0.5	63 ± 7	2.9 ± 0.3
Untreated primed cells	64 ± 64	2672 ± 68	36.6 ± 1.0	181 ± 103	2.5 ± 1.4
PDP-treated primed cells	73 ± 12	650 ± 42^*,^†	10.2 ± 0.7^*,^†	144 ± 11	2.3 ± 0.2

Spleen cells from C57BL/6 mice were first primed with BALB/c spleen cells in a 5-day mixed lymphocyte culture. The primed cells were then treated with photodynamic cell therapy. After treatment, the cells were incubated with a variety of irradiated stimulators for 42 hours. Frequencies of antigen-specific IFNγ-secreting cells were determined by enzyme-linked immunospot (ELISPOT) assay. The values represent mean ± SD of triplicates per 10⁶ spleen cells. The experiments were repeated twice. SI indicates stimulation index; PDP, photodynamic cell purging process.

P < .001, compared with untreated primed cells.

†

P < .05, compared with naı̈ve C57BL/6.

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