Expansion of bone marrow pro–B cells in 4 independently derived mouse lines bearing an Eμ-A1 transgene
| Line . | N . | IgM+B . | Pre-B . | Pro-B . | Pre-B/Pro-B . | |||
|---|---|---|---|---|---|---|---|---|
| % . | Cells (× 10−6) . | % . | Cells (× 10−6) . | % . | Cells (× 10−6) . | |||
| Wild-type | 9 | 47 ± 2.0 | 2.0 ± 0.93 | 46 ± 13 | 2.5 ± 0.15 | 6.7 ± 2.4 | 0.41 ± 0.21 | 7.4 |
| 8 | 8 | 41 ± 8 | 1.5 ± 0.61 | 34 ± 10 | 1.3 ± 0.57 | 25 ± 3.4 | 0.94 ± 0.44 | 1.4 |
| 6 | 2 | 39 | ND | 42 | ND | 19 | ND | 2.3 |
| 14 | 2 | 58 | ND | 28 | ND | 10 | ND | 2.8 |
| 95 | 5 | 40 ± 5.3 | ND | 47 ± 7.2 | ND | 9.8 ± 3.1 | ND | 5.5 |
| Line . | N . | IgM+B . | Pre-B . | Pro-B . | Pre-B/Pro-B . | |||
|---|---|---|---|---|---|---|---|---|
| % . | Cells (× 10−6) . | % . | Cells (× 10−6) . | % . | Cells (× 10−6) . | |||
| Wild-type | 9 | 47 ± 2.0 | 2.0 ± 0.93 | 46 ± 13 | 2.5 ± 0.15 | 6.7 ± 2.4 | 0.41 ± 0.21 | 7.4 |
| 8 | 8 | 41 ± 8 | 1.5 ± 0.61 | 34 ± 10 | 1.3 ± 0.57 | 25 ± 3.4 | 0.94 ± 0.44 | 1.4 |
| 6 | 2 | 39 | ND | 42 | ND | 19 | ND | 2.3 |
| 14 | 2 | 58 | ND | 28 | ND | 10 | ND | 2.8 |
| 95 | 5 | 40 ± 5.3 | ND | 47 ± 7.2 | ND | 9.8 ± 3.1 | ND | 5.5 |
Bone marrow cells from mice 4 to 8 weeks of age were analyzed using 3-color flow cytometry for the expression of B220, IgM, and CD43. Populations were expressed as a percentage of B220+ cells, including IgM+ B cells, IgM−CD43−pre–B cells, and IgM-CD43+ pro–B cells. In WT and line 8 mice, absolute cell numbers contained in 2 femurs are also given. Ratios of pre– to pro–B cells are also given. Mean ± SD are given for the indicated number of mice analyzed, except for lines 6 and 14, in which only 2 mice were studied. ND indicates not done.