Intrathymic development of CFSE-labeled CD34+thymocytes
| . | CD34+ thymocytes (it) . | CD34+ thymocytes (iv) . | ||
|---|---|---|---|---|
| CFSE− (%) . | CFSE+(%) . | CFSE− (%) . | CFSE+ (%) . | |
| CD4+CD8+ | 90.0 | 90.0 | 87.0 | 82.0 |
| CD1−CD34+ | 1.5 | 1.0 | ND | ND |
| CD1+CD34+ | 0.4 | 0.06 | ND | ND |
| CD123+CD45RA+ | 0.02 | 18.0 | 0.03 | 16.0 |
| . | CD34+ thymocytes (it) . | CD34+ thymocytes (iv) . | ||
|---|---|---|---|---|
| CFSE− (%) . | CFSE+(%) . | CFSE− (%) . | CFSE+ (%) . | |
| CD4+CD8+ | 90.0 | 90.0 | 87.0 | 82.0 |
| CD1−CD34+ | 1.5 | 1.0 | ND | ND |
| CD1+CD34+ | 0.4 | 0.06 | ND | ND |
| CD123+CD45RA+ | 0.02 | 18.0 | 0.03 | 16.0 |
CD34+ fetal thymocytes were labeled with CFSE and injected intravenously (iv) or intrathymically (it) into RAG2−/− γc−/− transplanted with a human fetal thymus liver graft. The expression of cell surface antigens was determined in the thymus graft 4 weeks after injection, as described in the Table 1 footnote. ND indicates not done.