Development of CFSE-labeled CD34+ fetal liver cells after injection into transplanted RAG2−/−γc−/− mice
| . | CD34+it . | CD34+ iv . | ||
|---|---|---|---|---|
| CFSE−(%) . | CFSE+ (%) . | CFSE−(%) . | CFSE+ (%) . | |
| CD4+CD8+ | 79.0 | 80 | 86.0 | 80 |
| CD1−CD34+ | 0.05 | 0 | 0.15 | 0 |
| CD1+CD34+ | 0.17 | 1 | 0.38 | 2 |
| CD123+CD45RA+ | 0.03 | 6 | 0.02 | 6 |
| . | CD34+it . | CD34+ iv . | ||
|---|---|---|---|---|
| CFSE−(%) . | CFSE+ (%) . | CFSE−(%) . | CFSE+ (%) . | |
| CD4+CD8+ | 79.0 | 80 | 86.0 | 80 |
| CD1−CD34+ | 0.05 | 0 | 0.15 | 0 |
| CD1+CD34+ | 0.17 | 1 | 0.38 | 2 |
| CD123+CD45RA+ | 0.03 | 6 | 0.02 | 6 |
CD34+ fetal liver cells were obtained by MACS sorting only (experiment 2), labeled with CFSE and injected intrathymically (it) or intravenously (iv) into RAG2−/−γc−/− transplanted with a human fetal thymus liver graft. The expression of cell surface antigens was determined in the thymus graft 4 weeks after injection using PE-labeled antibodies CD4, CD123, CD1a, and the TC-labeled antibodies CD8, CD34, and CD45RA. Isotype control antibodies were used to set the cursors. Electronic gates were placed on CFSE+ and CFSE− cells, and the percentages of different subpopulations were determined in each gate and are indicated in the table.