Table 1. Development of CFSE-labeled... | American Society of Hematology

Table 1.

Development of CFSE-labeled CD34⁺ fetal liver cells after injection into the human thymus transplanted in RAG2^−/− γc^−/− mice

	CD34⁺38⁻ it
	CFSE⁻ (%)	CFSE⁺ (%)
CD4⁺CD8⁺	85.0	87.0
CD1⁻CD34⁺	0.3	0.2
CD1⁺CD34⁺	0.5	2.0
CD123⁺CD45RA⁺	0.05	8.0

	CD34⁺38⁻ it
CD4⁺CD8⁺	85.0	87.0
CD1⁻CD34⁺	0.3	0.2
CD1⁺CD34⁺	0.5	2.0
CD123⁺CD45RA⁺	0.05	8.0

CD34⁺ fetal liver cells were obtained by FACS sorting (CD34⁺CD38⁻, 99% pure, experiment 1), labeled with CFSE and injected intrathymically (it) into RAG2^−/−γc^−/− transplanted with a human fetal thymus liver graft. The expression of cell surface antigens was determined in the thymus graft 6 weeks after injection using PE-labeled antibodies CD4, CD123, CD1a, and the TC-labeled antibodies CD8, CD34, and CD45RA. Isotype control antibodies were used to set the cursors. Electronic gates were placed on CFSE⁺ and CFSE⁻ cells, and the percentages of different subpopulations were determined in each gate and are indicated in the table.

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