Results of analysis of colony-stimulating activity in the patient's serum
| Stimulus . | GM-CFCs . | Meg-CFCs . | Erythroid colonies . | |||
|---|---|---|---|---|---|---|
| Donor 1 . | Donor 2 . | Donor 1 . | Donor 2 . | Donor 1 . | Donor 2 . | |
| None | 0 | 0 | 0 | 0 | 0 | 0 |
| None + patient's serum | 7 ± 1 | 5 ± 2 | 0 | 0 | 2 ± 1 | 15 ± 4 |
| Growth factors | 30 ± 2 | 21 ± 1 | 2.7 ± 0.2 | 1.7 ± 0.1 | 34 ± 1 | 58 ± 1 |
| Growth factors + patient's serum | 44 ± 4 | 60 ± 3 | 1.6 ± 0.1 | 4.2 ± 0.2 | 51 ± 5 | 65 ± 2 |
| Stimulus . | GM-CFCs . | Meg-CFCs . | Erythroid colonies . | |||
|---|---|---|---|---|---|---|
| Donor 1 . | Donor 2 . | Donor 1 . | Donor 2 . | Donor 1 . | Donor 2 . | |
| None | 0 | 0 | 0 | 0 | 0 | 0 |
| None + patient's serum | 7 ± 1 | 5 ± 2 | 0 | 0 | 2 ± 1 | 15 ± 4 |
| Growth factors | 30 ± 2 | 21 ± 1 | 2.7 ± 0.2 | 1.7 ± 0.1 | 34 ± 1 | 58 ± 1 |
| Growth factors + patient's serum | 44 ± 4 | 60 ± 3 | 1.6 ± 0.1 | 4.2 ± 0.2 | 51 ± 5 | 65 ± 2 |
Shown are results from replicate cultures using bone marrow samples from 2 healthy donors. Cells (104/mL) were incubated with no stimulus, 10% patient's serum, a combination of growth factors (granulocyte-macrophage [GM] colony-stimulating factor, granulocyte colony-stimulating factor, erythropoietin, interleukin 6, stem-cell factor, and pegylated recombinant human megakaryocyte growth and development factor), or a mixture of these stimuli. Erythroid-stimulating activity and GM-stimulating activity were present in the patient's serum samples.
GM-CFCs indicates GM colony-forming cells; Meg-CFCs, megakaryocyte colony-forming cells.