Intensity of expression of various surface and cytoplasmic antigens on the 3 DC subsets
| mAb . | CD4−/8− . | CD4+ . | CD8+ . | Clone . | Isotype . | Source . |
|---|---|---|---|---|---|---|
| Lineage | ||||||
| *CD1d | −↑ | +↑ | −↑ | 1B1 | Rat IgG2b | Pharmingen |
| CD3 | − | − | − | 17A2 | Rat IgG2b | Pharmingen |
| *CD4 | − | ++↓ | − | GK1.5 | Rat IgG2b | SBA |
| *CD8α | − | − | ++ | 53-6.7 | Rat IgG2a | ATCC |
| CD8β | − | − | − | 53-5-8 | Rat IgG1 | Pharmingen |
| CD13 | ++ | ++ | ++ | R3-242 | Rat IgG1 | Pharmingen |
| CD45 | ++ | ++ | ++ | 30-F11 | Rat IgG2b | Pharmingen |
| Thy 1.2 | − | − | − | 30-H12 | Rat IgG2b | ATCC |
| CD11b | + | + | − | M1/70 | Rat IgG2b | ATCC |
| CD19 | − | − | − | 1D3 | Rat IgG2a | ATCC |
| B220 | −/+ | −/+ | −/+ | RA3-6B2 | Rat IgG2a | Pharmingen |
| Ly49G | − | − | − | 4D11 | Rat IgG2b | ATCC |
| Gr-1 | − | − | − | RB6-8C5 | Rat IgG2b | ATCC |
| Dendritic cell | ||||||
| CD11c | ++ | ++ | ++ | N418 | Ham IgG | ATCC |
| *Langerin | − | − | +++ | 929F3 | rat IgG1 | S Saeland |
| 4F7 | − | − | − | 4F7 | Rat IgG1 | Knop |
| CD205 (DEC-205) | +↑ | +↑ | ++ | NLDC-145 | Rat IgG2a | G Kraal |
| 2A1 | ++ | ++ | ++ | 2A1 | Rat IgG2a | R Steinman |
| *33D1 | + | + | − | 33D1 | Rat IgG2b | ATCC |
| MIDC-8 | + | + | + | MIDC-8 | Rat IgG2a | G Kraal |
| Macrophage | ||||||
| *F4/80 (red pulp Mφ) | +/− | + | − | F4/80 | Rat IgG2b | S Gordon |
| CD68 (macrosialin) | + | + | + | FA-11 | Rat IgG2a | S Gordon |
| ERTR-9 (marginal zone Mφ) | − | − | − | ERTR-9 | Rat IgM | G Kraal |
| MOMA-1 (metallophillic Mφ) | − | − | − | MOMA-1 | Rat IgG2a | G Kraal |
| MOMA-2 | − | − | − | MOMA-2 | Rat IgG2b | G Kraal |
| MAC-3 | − | − | − | M3/84 | Rat IgG1 | Pharmingen |
| MHC | ||||||
| Class I | ++↑ | ++↑ | ++↑ | M1/42 | Rat IgG2a | ATCC |
| Class II | ++↑ | ++↑ | ++↑ | M5/114 | Rat IgG2b | ATCC |
| CD74 (inv chain) | ++ | ++ | ++ | IN-1 | Rat IgG2b | N Koch |
| Costimulator | ||||||
| *CD24 | + | + | ++ | J11d | Rat IgM | Pharmingen |
| CD40 | +↑ | +↑ | +↑ | FGK-45 | Rat IgG | A Rolink |
| CD80 | −/+↑ | −/+↑ | −/+↑ | 1G10 | Rat IgG2a | Pharmingen |
| CD86 | +↑ | +↑ | +↑ | GL-1 | Rat IgG2a | ATCC |
| OX40L | − | − | − | RM134L | Rat IgG2b | Pharmingen |
| 4-1BBL | − | − | − | 1AH2 | Rat IgG1 | Pharmingen |
| Adhesion/homing | ||||||
| CD11a | ++ | ++ | ++ | FD441.8 | Rat IgG2b | ATCC |
| CD18 | +++ | +++ | +++ | C71/16 | Rat IgG2a | Pharmingen |
| CD29 | + | + | + | 9EG7 | Rat IgG2a | Pharmingen |
| *CD43 | ++ | + | + | S7 | Rat IgG2a | Pharmingen |
| *CD44 | +↑ | +↑ | ++↑ | KM201 | Rat IgG1 | SBA |
| *CD44H | ++ | ++ | +++ | TM-1 | Ham IgG | Pharmingen |
| CD54 (ICAM-1) | +↑ | +↑ | +↑ | KAT-1 | Rat IgG2a | SBA |
| *CD62L | + | + | ++ | MEL-14 | Rat IgG2a | Pharmingen |
| *CD49a (α1integrin) | −/+ | + | −/+ | Ha31/8 | Ham IgG | Pharmingen |
| CD49d (α4 integrin) | + | + | + | PS/2 | Rat IgG2b | SBA |
| *CD49e (α5integrin) | ++ | + | ++ | 5H10-27 | Rat IgG2a | Pharmingen |
| *CD49f (α6integrin) | + | + | ++ | GoH3 | Rat IgG2a | Pharmingen |
| CD61 (β3integrin) | − | − | − | 2C9G2 | Ham IgG | Pharmingen |
| *β7 integrin | + | + | ++ | M293 | Rat IgG2a | Pharmingen |
| CD102 (ICAM-2) | + | + | + | 3C4 | Rat IgG2a | Pharmingen |
| *CD103 (αIEL-integrin) | − | − | ++ | M290 | Rat IgG2a | Pharmingen |
| *CD162 (PSGL-1) | ++ | ++ | +++ | 2PH1 | Rat IgG1 | Pharmingen |
| CLA | − | − | − | HECA-452 | Rat IgM | Pharmingen |
| *α4β7(LPAM-1) | −/+ | + | − | DATK32 | Rat IgG2a | Pharmingen |
| CD106 (VCAM-1) | −/+ | −/+ | −/+ | 429 | Rat IgG2a | Pharmingen |
| mAb | CD4−/8− | CD4+ | CD8+ | Clone | Isotype | Source |
| Heat shock proteins | ||||||
| hsp70 ind | − | − | − | C92F3A-5 | Mu IgG1 | StressGen |
| hsp70 ind/con | + | + | + | N27F3-4 | Mu IgG1 | StressGen |
| hsp70 con | ++ | ++ | ++ | 1B5 | Rat IgG2a | StressGen |
| hsp90 | + | + | + | 16F1 | Rat IgG2a | StressGen |
| grp94 | ++ | ++ | ++ | 9G10 | Rat IgG2a | StressGen |
| Cytokine receptor | ||||||
| IL-4R | −↑ | −↑ | −↑ | M1 | Rat IgG2a | Genzyme |
| c-kit (CD117) | −↑ | −↑ | −↑ | 3C1 | Rat IgG2a | Pharmingen |
| *sca-1 | +↑ | +↑ | ++↑ | E13-161.7 | Rat IgG2a | Pharmingen |
| CD25 (IL-2Rα) | −↑ | −↑ | −↑ | 7D4 | Rat IgM | ATCC |
| CD123 (IL-3R) | −↑ | −↑ | −↑ | 5B11 | Rat IgG2a | Pharmingen |
| IFN-γR β-chain | + | + | + | MOB47 | Ham IgG | Genzyme |
| Miscellaneous | ||||||
| CD16/32 | − | − | − | 2.4G2 | Rat IgG2b | ATCC |
| CD81 (TAPA) | − | − | − | 2F7 | Ham IgG | Pharmingen |
| CD95 | − | − | − | RMF2 | Rat IgG1 | Coulter-Immunotech |
| CD95L | − | − | − | MFL3 | Ham IgG | Pharmingen |
| mAb . | CD4−/8− . | CD4+ . | CD8+ . | Clone . | Isotype . | Source . |
|---|---|---|---|---|---|---|
| Lineage | ||||||
| *CD1d | −↑ | +↑ | −↑ | 1B1 | Rat IgG2b | Pharmingen |
| CD3 | − | − | − | 17A2 | Rat IgG2b | Pharmingen |
| *CD4 | − | ++↓ | − | GK1.5 | Rat IgG2b | SBA |
| *CD8α | − | − | ++ | 53-6.7 | Rat IgG2a | ATCC |
| CD8β | − | − | − | 53-5-8 | Rat IgG1 | Pharmingen |
| CD13 | ++ | ++ | ++ | R3-242 | Rat IgG1 | Pharmingen |
| CD45 | ++ | ++ | ++ | 30-F11 | Rat IgG2b | Pharmingen |
| Thy 1.2 | − | − | − | 30-H12 | Rat IgG2b | ATCC |
| CD11b | + | + | − | M1/70 | Rat IgG2b | ATCC |
| CD19 | − | − | − | 1D3 | Rat IgG2a | ATCC |
| B220 | −/+ | −/+ | −/+ | RA3-6B2 | Rat IgG2a | Pharmingen |
| Ly49G | − | − | − | 4D11 | Rat IgG2b | ATCC |
| Gr-1 | − | − | − | RB6-8C5 | Rat IgG2b | ATCC |
| Dendritic cell | ||||||
| CD11c | ++ | ++ | ++ | N418 | Ham IgG | ATCC |
| *Langerin | − | − | +++ | 929F3 | rat IgG1 | S Saeland |
| 4F7 | − | − | − | 4F7 | Rat IgG1 | Knop |
| CD205 (DEC-205) | +↑ | +↑ | ++ | NLDC-145 | Rat IgG2a | G Kraal |
| 2A1 | ++ | ++ | ++ | 2A1 | Rat IgG2a | R Steinman |
| *33D1 | + | + | − | 33D1 | Rat IgG2b | ATCC |
| MIDC-8 | + | + | + | MIDC-8 | Rat IgG2a | G Kraal |
| Macrophage | ||||||
| *F4/80 (red pulp Mφ) | +/− | + | − | F4/80 | Rat IgG2b | S Gordon |
| CD68 (macrosialin) | + | + | + | FA-11 | Rat IgG2a | S Gordon |
| ERTR-9 (marginal zone Mφ) | − | − | − | ERTR-9 | Rat IgM | G Kraal |
| MOMA-1 (metallophillic Mφ) | − | − | − | MOMA-1 | Rat IgG2a | G Kraal |
| MOMA-2 | − | − | − | MOMA-2 | Rat IgG2b | G Kraal |
| MAC-3 | − | − | − | M3/84 | Rat IgG1 | Pharmingen |
| MHC | ||||||
| Class I | ++↑ | ++↑ | ++↑ | M1/42 | Rat IgG2a | ATCC |
| Class II | ++↑ | ++↑ | ++↑ | M5/114 | Rat IgG2b | ATCC |
| CD74 (inv chain) | ++ | ++ | ++ | IN-1 | Rat IgG2b | N Koch |
| Costimulator | ||||||
| *CD24 | + | + | ++ | J11d | Rat IgM | Pharmingen |
| CD40 | +↑ | +↑ | +↑ | FGK-45 | Rat IgG | A Rolink |
| CD80 | −/+↑ | −/+↑ | −/+↑ | 1G10 | Rat IgG2a | Pharmingen |
| CD86 | +↑ | +↑ | +↑ | GL-1 | Rat IgG2a | ATCC |
| OX40L | − | − | − | RM134L | Rat IgG2b | Pharmingen |
| 4-1BBL | − | − | − | 1AH2 | Rat IgG1 | Pharmingen |
| Adhesion/homing | ||||||
| CD11a | ++ | ++ | ++ | FD441.8 | Rat IgG2b | ATCC |
| CD18 | +++ | +++ | +++ | C71/16 | Rat IgG2a | Pharmingen |
| CD29 | + | + | + | 9EG7 | Rat IgG2a | Pharmingen |
| *CD43 | ++ | + | + | S7 | Rat IgG2a | Pharmingen |
| *CD44 | +↑ | +↑ | ++↑ | KM201 | Rat IgG1 | SBA |
| *CD44H | ++ | ++ | +++ | TM-1 | Ham IgG | Pharmingen |
| CD54 (ICAM-1) | +↑ | +↑ | +↑ | KAT-1 | Rat IgG2a | SBA |
| *CD62L | + | + | ++ | MEL-14 | Rat IgG2a | Pharmingen |
| *CD49a (α1integrin) | −/+ | + | −/+ | Ha31/8 | Ham IgG | Pharmingen |
| CD49d (α4 integrin) | + | + | + | PS/2 | Rat IgG2b | SBA |
| *CD49e (α5integrin) | ++ | + | ++ | 5H10-27 | Rat IgG2a | Pharmingen |
| *CD49f (α6integrin) | + | + | ++ | GoH3 | Rat IgG2a | Pharmingen |
| CD61 (β3integrin) | − | − | − | 2C9G2 | Ham IgG | Pharmingen |
| *β7 integrin | + | + | ++ | M293 | Rat IgG2a | Pharmingen |
| CD102 (ICAM-2) | + | + | + | 3C4 | Rat IgG2a | Pharmingen |
| *CD103 (αIEL-integrin) | − | − | ++ | M290 | Rat IgG2a | Pharmingen |
| *CD162 (PSGL-1) | ++ | ++ | +++ | 2PH1 | Rat IgG1 | Pharmingen |
| CLA | − | − | − | HECA-452 | Rat IgM | Pharmingen |
| *α4β7(LPAM-1) | −/+ | + | − | DATK32 | Rat IgG2a | Pharmingen |
| CD106 (VCAM-1) | −/+ | −/+ | −/+ | 429 | Rat IgG2a | Pharmingen |
| mAb | CD4−/8− | CD4+ | CD8+ | Clone | Isotype | Source |
| Heat shock proteins | ||||||
| hsp70 ind | − | − | − | C92F3A-5 | Mu IgG1 | StressGen |
| hsp70 ind/con | + | + | + | N27F3-4 | Mu IgG1 | StressGen |
| hsp70 con | ++ | ++ | ++ | 1B5 | Rat IgG2a | StressGen |
| hsp90 | + | + | + | 16F1 | Rat IgG2a | StressGen |
| grp94 | ++ | ++ | ++ | 9G10 | Rat IgG2a | StressGen |
| Cytokine receptor | ||||||
| IL-4R | −↑ | −↑ | −↑ | M1 | Rat IgG2a | Genzyme |
| c-kit (CD117) | −↑ | −↑ | −↑ | 3C1 | Rat IgG2a | Pharmingen |
| *sca-1 | +↑ | +↑ | ++↑ | E13-161.7 | Rat IgG2a | Pharmingen |
| CD25 (IL-2Rα) | −↑ | −↑ | −↑ | 7D4 | Rat IgM | ATCC |
| CD123 (IL-3R) | −↑ | −↑ | −↑ | 5B11 | Rat IgG2a | Pharmingen |
| IFN-γR β-chain | + | + | + | MOB47 | Ham IgG | Genzyme |
| Miscellaneous | ||||||
| CD16/32 | − | − | − | 2.4G2 | Rat IgG2b | ATCC |
| CD81 (TAPA) | − | − | − | 2F7 | Ham IgG | Pharmingen |
| CD95 | − | − | − | RMF2 | Rat IgG1 | Coulter-Immunotech |
| CD95L | − | − | − | MFL3 | Ham IgG | Pharmingen |
The levels of expression of various surface and cytoplasmic markers on the three DC subsets are represented on a scale from − to +++ (MFI above background staining of − = 0, −/+ = 10-50, + = 50-100, ++ =. 100-500, +++ = 500-10 000). Arrows refer to increases in antigen expression detected following overnight culture and asterisks highlight markers showing differential expression on the DC subsets. For the heat shock protein expression, ind indicates inducible, and con, constitutive.
SBA indicates Southern Biotechnology Associates; ATCC, American Type Culture Collection; ham, hamster; mu, murine; MHC, major histocompatibility complex; inv, invariant chain; ICAM-1, intercellular adhesion molecule-1; PSGL-1, P-selectin glycoprotein ligand-1; CLA, cutaneous lymphocyte antigen; LPAM-1, lymphocyte Peyer patch adhesion molecule; VCAM-1, vascular cell adhesion molecule 1; TAPA, target of antiproliferative antibody.