Table 1. Comparison of endothelial and... | American Society of Hematology

Table 1.

Comparison of endothelial and nonendothelial mitotic indices in wild type and flt-1^−/− embryonic stem cell cultures

	Cell no.^*		Mitotic index^†		EI/NEI × 100^1-155
	Endothelial^‡	Nonendothelial^1-153	Endothelial	Nonendothelial	EI/NEI × 100^1-155
Day 6
WT Exp 1	1 543	4 006	2.31	1.61	143.5
^−/−Exp 1	2 719	2 052	4.02	1.22	329.5
WT Exp 2	1 050	3 060	2.66	1.99	133.7
^−/−Exp 2	1 072	2 374	6.14	1.32	465.2
Day 7
WT Exp 1	3 554	11 303	1.88	1.78	105.6
^−/−Exp 1	4 797	7 820	2.83	2.10	134.8
WT Exp 2	1 777	6 498	1.18	1.24	95.2
^−/−Exp 2	3 216	4 703	3.25	1.02	318.6

	Cell no.^*		Mitotic index^†		EI/NEI × 100^1-155
	Endothelial^‡	Nonendothelial^1-153	Endothelial	Nonendothelial	EI/NEI × 100^1-155
Day 6
WT Exp 1	1 543	4 006	2.31	1.61	143.5
^−/−Exp 1	2 719	2 052	4.02	1.22	329.5
WT Exp 2	1 050	3 060	2.66	1.99	133.7
^−/−Exp 2	1 072	2 374	6.14	1.32	465.2
Day 7
WT Exp 1	3 554	11 303	1.88	1.78	105.6
^−/−Exp 1	4 797	7 820	2.83	2.10	134.8
WT Exp 2	1 777	6 498	1.18	1.24	95.2
^−/−Exp 2	3 216	4 703	3.25	1.02	318.6

EI, endothelial index; NEI, nonendothelial index; WT, wild type; Exp, experiment.

Total number of nuclei counted.

Percentage of replicating cells as determined by labeling with the mitotic marker phosphohistone H3.

Number of nuclei with PECAM-labeling along the cell border.

Nuclei of cells that did not exhibit PECAM-labeling.

Formula used to express each endothelial index as a percentage of its companion nonendothelial index.