CD11b and CD18 expression at the surface of resting and fMLP-stimulated PMNs from controls and patients
Subjects . | MFI . | |||
---|---|---|---|---|
Unstimulated PMNs . | fMLP-stimulated PMNs . | |||
CD11b . | CD18 . | CD11b . | CD18 . | |
African controls (n = 11) | 242 ± 30 | 47 ± 4 | 2957 ± 130 | 333 ± 23 |
Non-VOC group (n = 15) | 226 ± 22 | 49 ± 5 | 2356 ± 1483-150 | 240 ± 253-150 |
VOC group (n = 17) | 227 ± 15 | 44 ± 3 | 2545 ± 160 | 266 ± 173-150 |
HU group (n = 9) | 247 ± 40 | 49 ± 3 | 2425 ± 1603-150 | 259 ± 193-150 |
Subjects . | MFI . | |||
---|---|---|---|---|
Unstimulated PMNs . | fMLP-stimulated PMNs . | |||
CD11b . | CD18 . | CD11b . | CD18 . | |
African controls (n = 11) | 242 ± 30 | 47 ± 4 | 2957 ± 130 | 333 ± 23 |
Non-VOC group (n = 15) | 226 ± 22 | 49 ± 5 | 2356 ± 1483-150 | 240 ± 253-150 |
VOC group (n = 17) | 227 ± 15 | 44 ± 3 | 2545 ± 160 | 266 ± 173-150 |
HU group (n = 9) | 247 ± 40 | 49 ± 3 | 2425 ± 1603-150 | 259 ± 193-150 |
Whole blood was incubated with anti-CD11b and anti-CD18 antibodies at 4°C for 30 minutes, either after maintaining the samples at 4°C (unstimulated PMNs) or after incubation with PBS or fMLP (10−6 M) at 37°C for 5 minutes. The MFI was recorded as described in “Patients, materials, and methods.” Values are given as means ± SEM.
Significantly different from controls (P < .05).