L Selectin expression at the surface of unstimulated and fMLP-stimulated PMNs from controls and patients
| Subjects . | MFI . | |
|---|---|---|
| Unstimulated PMNs . | fMLP-stimulated PMNs . | |
| African controls (n = 11) | 167 ± 18 | 68 ± 7 |
| Non-VOC group (n = 15) | 108 ± 12* | 78 ± 10 |
| VOC group (n = 17) | 113 ± 16* | 107 ± 16*,† |
| HU group (n = 9) | 99 ± 11* | 50 ± 4 |
| Subjects . | MFI . | |
|---|---|---|
| Unstimulated PMNs . | fMLP-stimulated PMNs . | |
| African controls (n = 11) | 167 ± 18 | 68 ± 7 |
| Non-VOC group (n = 15) | 108 ± 12* | 78 ± 10 |
| VOC group (n = 17) | 113 ± 16* | 107 ± 16*,† |
| HU group (n = 9) | 99 ± 11* | 50 ± 4 |
Whole blood was incubated with anti-CD62L antibody at 4°C for 30 minutes either after maintaining the samples at 4°C (unstimulated PMNs) or after incubation with PBS or fMLP (10−6 M) at 37°C for 5 minutes. The MFI was recorded as described in “Patients, materials, and methods.” Values obtained after 5 minutes of incubation with PBS at 37°C did not differ from that obtained after maintaining the samples at 4°C. Values are given as means ± SEM.
Significantly different from controls (P < .05).
Significantly different from HU-treated patients (P < .05).