Oligonucleotides used for mutagenesis and electrophoretic mobility shift assay
| Name . | Sequence . | Position . |
|---|---|---|
| wt1 | 5′-gcacacacacacacacacacatgcctcagcaagtccc-3′ | −175/−139 |
| mut1 | 5′-gcacacacacacacacacttaatcctcagcaagtccc-3′ | |
| wt2 | 5′-cctgaatgctcttacacacgtacacacacagagcagc-3′ | −210/−174 |
| mut2 | 5′-cctgaatgctcttacacttaatcacacacagagcagc-3′ | |
| wt3 | 5′-gccctgggggaaaacttccacgttttgatggaggttatc-3′ | −471/−433 |
| mut3 | 5′-gccctgggggaaaacttccttaatttgatggaggttatc-3′ | |
| wt4 | 5′-cagacaaaacctagacaatcacgtggctggctgcattgccc-3′ | −585/−545 |
| mut4 | 5′-cagacaaaacctagacaatcttaatgctggctgcattgccc-3′ | |
| wt5 | 5′-ggggcacagagagagtctggacacgtggggagtcagccg-3′ | −703/−664 |
| mut5 | 5′-ggggcacagagagagtctggacttaatgggagtcagccg-3′ |
| Name . | Sequence . | Position . |
|---|---|---|
| wt1 | 5′-gcacacacacacacacacacatgcctcagcaagtccc-3′ | −175/−139 |
| mut1 | 5′-gcacacacacacacacacttaatcctcagcaagtccc-3′ | |
| wt2 | 5′-cctgaatgctcttacacacgtacacacacagagcagc-3′ | −210/−174 |
| mut2 | 5′-cctgaatgctcttacacttaatcacacacagagcagc-3′ | |
| wt3 | 5′-gccctgggggaaaacttccacgttttgatggaggttatc-3′ | −471/−433 |
| mut3 | 5′-gccctgggggaaaacttccttaatttgatggaggttatc-3′ | |
| wt4 | 5′-cagacaaaacctagacaatcacgtggctggctgcattgccc-3′ | −585/−545 |
| mut4 | 5′-cagacaaaacctagacaatcttaatgctggctgcattgccc-3′ | |
| wt5 | 5′-ggggcacagagagagtctggacacgtggggagtcagccg-3′ | −703/−664 |
| mut5 | 5′-ggggcacagagagagtctggacttaatgggagtcagccg-3′ |
Only the sequences of the sense oligonucleotides are shown. For mutagenesis and electrophoretic mobility shift assay, the complementary probes were used. The putative hypoxia-response elements are shown in boldface type, and the mutations are underlined.