Suppression of cytokine production of alloantigen-specific CD4+ T cells and tyrosinase-specific CD8+ T cells after coculture with anergic T cells
| . | Cytokine production, pg/mL . | |||
|---|---|---|---|---|
| IL-2 . | IFN-γ . | IL-4 . | IL-10 . | |
| Alloantigen-specific CD4+ T cells | ||||
| Activated | 2887 ± 122 | 4906 ± 81 | 38 ± 14 | nd |
| Anergic | 302 ± 63 | 482 ± 18 | 18 ± 10 | nd |
| Cocultured | 482 ± 101 | 372 ± 97 | 21 ± 15 | nd |
| Tyrosinase-specific CD8+ T cells | ||||
| Activated | 1705 ± 120 | 3768 ± 182 | 29 ± 14 | nd |
| Anergic | 104 ± 55 | 821 ± 65 | nd | nd |
| Cocultured | 287 ± 156 | 841 ± 34 | 17 ± 13 | nd |
| . | Cytokine production, pg/mL . | |||
|---|---|---|---|---|
| IL-2 . | IFN-γ . | IL-4 . | IL-10 . | |
| Alloantigen-specific CD4+ T cells | ||||
| Activated | 2887 ± 122 | 4906 ± 81 | 38 ± 14 | nd |
| Anergic | 302 ± 63 | 482 ± 18 | 18 ± 10 | nd |
| Cocultured | 482 ± 101 | 372 ± 97 | 21 ± 15 | nd |
| Tyrosinase-specific CD8+ T cells | ||||
| Activated | 1705 ± 120 | 3768 ± 182 | 29 ± 14 | nd |
| Anergic | 104 ± 55 | 821 ± 65 | nd | nd |
| Cocultured | 287 ± 156 | 841 ± 34 | 17 ± 13 | nd |
Control alloantigen-specific anergic and activated CD4+ T cells or tyrosinase-specific CD8+ T cells and the same number of cocultured T cells of these 2 cell populations were restimulated with mature dendritic cells. After 48 hours, the supernatants of the T-cell culture were harvested and assessed for IL-2, IFN-γ, IL-4, and IL-10 content by enzyme-linked immunosorbent assay.
IL indicates interleukin; IFN, interferon; nd, not detectable.