Migration across Fn of CD34+ cells fractionated on the basis of VLA-5 expression and cell cycle status
Experiment no. . | Cell fraction . | |||
---|---|---|---|---|
CD34+VLA-5low . | CD34+VLA-5high . | |||
G0/G1 . | S/G2 + M . | G0/G1 . | S/G2 + M . | |
1 | 39.0 | 24.5 | 38.2 | 17.2 |
2 | 43.6 | 26.7 | 35.8 | 27.1 |
3 | 40.3 | 19.9 | 40.0 | 22.7 |
Mean | 40.93-150 | 23.7 | 38.03-150 | 22.3 |
SEM | 1.4 | 2.0 | 1.2 | 2.9 |
Experiment no. . | Cell fraction . | |||
---|---|---|---|---|
CD34+VLA-5low . | CD34+VLA-5high . | |||
G0/G1 . | S/G2 + M . | G0/G1 . | S/G2 + M . | |
1 | 39.0 | 24.5 | 38.2 | 17.2 |
2 | 43.6 | 26.7 | 35.8 | 27.1 |
3 | 40.3 | 19.9 | 40.0 | 22.7 |
Mean | 40.93-150 | 23.7 | 38.03-150 | 22.3 |
SEM | 1.4 | 2.0 | 1.2 | 2.9 |
Cultured CD34+ cells were sorted as depicted in Figure 9 into 4 fractions. Data represent percent migration of each fraction across Fn toward MS-5 CM.
P < .05 compared with S/G2+ M in both VLA-5low and VLA-5high subsets.