Phenotype of the leukemic cell lines and primary leukemias described in this study
| Leukemic sample . | Flt-1 expression (%) . | KDR expression (%) . | VEGF (pg/mL) . |
|---|---|---|---|
| HL-60 | 7.92 ± 5.1 | 14.5 ± 5.4 | 144 ± 35 |
| HEL | 20 ± 3.9 | 6.1 ± 2.8 | 94 ± 15 |
| THP-1 | 6.8 ± 2.7 | 5 ± 2.3 | 90 ± 18 |
| 1 | 15.2 ± 2.9 | 10.4 ± 4.2 | 120 ± 16 |
| 2 | 8.8 ± 3.1 | — | 93 ± 21 |
| 3 | — | 7.3 ± 4.4 | 88 ± 18 |
| 4 | 21.4 ± 6.5 | 28 ± 8.1 | 196 ± 20 |
| Leukemic sample . | Flt-1 expression (%) . | KDR expression (%) . | VEGF (pg/mL) . |
|---|---|---|---|
| HL-60 | 7.92 ± 5.1 | 14.5 ± 5.4 | 144 ± 35 |
| HEL | 20 ± 3.9 | 6.1 ± 2.8 | 94 ± 15 |
| THP-1 | 6.8 ± 2.7 | 5 ± 2.3 | 90 ± 18 |
| 1 | 15.2 ± 2.9 | 10.4 ± 4.2 | 120 ± 16 |
| 2 | 8.8 ± 3.1 | — | 93 ± 21 |
| 3 | — | 7.3 ± 4.4 | 88 ± 18 |
| 4 | 21.4 ± 6.5 | 28 ± 8.1 | 196 ± 20 |
Cells were stained for surface VEGFR-1 (Flt-1) and VEGFR-2 (KDR), the level of surface expression determined by flow cytometry, as described in “Materials and methods,” and were also analyzed for VEGF production (by ELISA).
Dash indicates that there were no Flt-1+ or KDR+ cells detected in the total cell population.