Some characteristics of antiheparin antibodies
| Antibody . | CDR3 sequence . | VH family . | Germline segment . | Number of clones . |
|---|---|---|---|---|
| EW3D10 | GRTVGRN | 3 | 38 | 2 |
| EW3E4 | DRRNTQKTRYRT | 3 | 38 | 1 |
| EW3F5 | SGRQARQGRFPK | 3 | 38 | 1 |
| EW3G6 | GGTTRIRK | 3 | 38 | 8 |
| EW3H12 | GMRPRL | 3 | 38 | 3 |
| EW4A4 | GTKLKMTK | 1 | 8 | 2 |
| EW4A11 | ERNTIRR | 3 | 38 | 1 |
| EW4B5 | GRLHLPRK | 3 | 45 | 4 |
| EW4B7 | SSSRHHRLHR | 1 | 8 | 1 |
| EW4B10 | QRWKPAVTPKLV | 7 | 25 | 1 |
| EW4C10 | ARMTGHVRNVMI | 3 | 45 | 1 |
| EW4D2 | GKMKLNR | 3 | 38 | 1 |
| EW4D5 | PVSHRKWRVTV | 3 | 45 | 1 |
| EW4E1 | GRRHKLIR | 3 | 38 | 4 |
| EW4E9 | LRGTKMFRH | 3 | 38 | 1 |
| EW4E10 | SRKTPKPFMRK | 1 | 3 | 1 |
| EW4G1 | GARLKR | 3 | 42 | 1 |
| EW4G2 | GKVKLPN | 3 | 38 | 1 |
| EW4G10 | GTKKLGK | 3 | 45 | 1 |
| Antibody . | CDR3 sequence . | VH family . | Germline segment . | Number of clones . |
|---|---|---|---|---|
| EW3D10 | GRTVGRN | 3 | 38 | 2 |
| EW3E4 | DRRNTQKTRYRT | 3 | 38 | 1 |
| EW3F5 | SGRQARQGRFPK | 3 | 38 | 1 |
| EW3G6 | GGTTRIRK | 3 | 38 | 8 |
| EW3H12 | GMRPRL | 3 | 38 | 3 |
| EW4A4 | GTKLKMTK | 1 | 8 | 2 |
| EW4A11 | ERNTIRR | 3 | 38 | 1 |
| EW4B5 | GRLHLPRK | 3 | 45 | 4 |
| EW4B7 | SSSRHHRLHR | 1 | 8 | 1 |
| EW4B10 | QRWKPAVTPKLV | 7 | 25 | 1 |
| EW4C10 | ARMTGHVRNVMI | 3 | 45 | 1 |
| EW4D2 | GKMKLNR | 3 | 38 | 1 |
| EW4D5 | PVSHRKWRVTV | 3 | 45 | 1 |
| EW4E1 | GRRHKLIR | 3 | 38 | 4 |
| EW4E9 | LRGTKMFRH | 3 | 38 | 1 |
| EW4E10 | SRKTPKPFMRK | 1 | 3 | 1 |
| EW4G1 | GARLKR | 3 | 42 | 1 |
| EW4G2 | GKVKLPN | 3 | 38 | 1 |
| EW4G10 | GTKKLGK | 3 | 45 | 1 |
Given are the antibody code, amino acid sequence of the VH complementary-determining region 3 (CDR3), VH family, germline segment (DP numbering), and the number of clones identified. CDR3 sequences are shown in single-letter amino acid code. Q represents translation of amber codon to glutamine in E coli TG1 supE strain. VH families and DP segments were deduced from the V BASE using DNAPLOT alignment (http://www.mrc-cpe.cam.ac.uk/imt-doc/restricted/DNAPLOT.html) by applying the full-length VH sequences of the antiheparin antibody clones (nomenclature according to Tomlinson et al29).