Antigenic specificity, helper activity inducing anti-β2GPI antibody production, and TCRβ chain of β2GPI-specific CD4+ T-cell clones generated from APS patients
| T-cell clone . | Donor . | T-cell epiptope . | HLA restriction . | Helper activity . | Gene segment . | Amino acid sequence3-150 . | CDR3 length . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Vβ . | Jβ . | Cβ . | Vβ . | N-Dβ-N-Jβ . | Jβ . | ||||||
| KS3 | APS3 | p276-290 | DRB43-1500103 | + | 7.1 | 1.2 | 1 | CASS | QFWTGRNGGYTF | GSGTRLTVV | 12 |
| KS8 | APS3 | Domain V | HLA-DR | ND | 13.1 | 1.2 | 1 | CAS | ISSWTGSYGYTF | GSGTRLTVV | 12 |
| OM23-151 | APS4 | p276-290 | DRB43-1500103 | + | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 |
| OM73-151 | APS4 | p276-290 | DRB43-1500103 | + | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 |
| EY33-151 | —3-152 | p276-290 | DRB43-1500103 | + | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 |
| EY83-151 | —3-152 | p276-290 | DRB43-1500103 | + | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 |
| T-cell clone . | Donor . | T-cell epiptope . | HLA restriction . | Helper activity . | Gene segment . | Amino acid sequence3-150 . | CDR3 length . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Vβ . | Jβ . | Cβ . | Vβ . | N-Dβ-N-Jβ . | Jβ . | ||||||
| KS3 | APS3 | p276-290 | DRB43-1500103 | + | 7.1 | 1.2 | 1 | CASS | QFWTGRNGGYTF | GSGTRLTVV | 12 |
| KS8 | APS3 | Domain V | HLA-DR | ND | 13.1 | 1.2 | 1 | CAS | ISSWTGSYGYTF | GSGTRLTVV | 12 |
| OM23-151 | APS4 | p276-290 | DRB43-1500103 | + | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 |
| OM73-151 | APS4 | p276-290 | DRB43-1500103 | + | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 |
| EY33-151 | —3-152 | p276-290 | DRB43-1500103 | + | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 |
| EY83-151 | —3-152 | p276-290 | DRB43-1500103 | + | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 |
p276-290 indicates a peptide encompassing amino acid residues 276-290 of β2GPI; ND, not determined.
The TGxxN/Q motif in Vβ7+ and Vβ13.1+ TCR is underlined.
The TCRβ gene segment and the CDR3 amino acid sequence of these T cell clones are identical.
These T cell clones were generated from an APS patient in whom blood sample was unavailable for the PCR-SSCP analysis.