Vβ-Jβ-Cβ gene rearrangement, CDR3 amino acid sequence, and CDR3 length in TCRβ chains used by β2GPI-reactive T cells
| Vβ family . | Donor . | Gene segment* . | Amino acid sequence† . | CDR3 length (amino acids) . | ||||
|---|---|---|---|---|---|---|---|---|
| Vβ . | Jβ . | Cβ . | Vβ . | N-Dβ-N-Jβ . | Jβ . | |||
| Vβ7 | APS2 | 7.1 | 1.5 | 1 | CASS | QAGRTGGDQPQHF | GDGTRLSIL | 13 |
| APS2‡ | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 | |
| APS3 | 7.1 | 1.2 | 1 | CASS | QFWTGRNGGYTF | GSGTRLTVV | 12 | |
| APS4‡ | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 | |
| APS12‡ | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 | |
| HD4 | 7.2 | 1.6 | 1 | CASS | QDLETGGSNYNSPLHF | GNGTRLTVT | 16 | |
| HD11 | 7.1 | 1.2 | 1 | CASS | QDTGGSNYGYTF | GSGTRLTVV | 12 | |
| Vβ8 | APS12 | 8.1 | 2.3 | 2 | CAS | RPSAGADTQYF | GPGTRLTVL | 11 |
| HD2 | 8.2 | 2.7 | 2 | CASS | PRADYEQYF | GPGTRLTVT | 9 | |
| HD4 | 8.1 | 2.3 | 2 | CAS | GPSAGADTQYF | GPGTRLTVL | 11 | |
| Vβ13.1 | APS3 | 13.1 | 1.2 | 1 | CAS | ISSWTGSYGYTF | GSGTRLTVV | 12 |
| HD4 | 13.1 | 1.5 | 1 | CASS | PGTGSNQPQHF | GDGTRLSIL | 11 | |
| Vβ family . | Donor . | Gene segment* . | Amino acid sequence† . | CDR3 length (amino acids) . | ||||
|---|---|---|---|---|---|---|---|---|
| Vβ . | Jβ . | Cβ . | Vβ . | N-Dβ-N-Jβ . | Jβ . | |||
| Vβ7 | APS2 | 7.1 | 1.5 | 1 | CASS | QAGRTGGDQPQHF | GDGTRLSIL | 13 |
| APS2‡ | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 | |
| APS3 | 7.1 | 1.2 | 1 | CASS | QFWTGRNGGYTF | GSGTRLTVV | 12 | |
| APS4‡ | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 | |
| APS12‡ | 7.2 | 1.2 | 1 | CASS | HDTGASNYGYTF | GSGTRLTVV | 12 | |
| HD4 | 7.2 | 1.6 | 1 | CASS | QDLETGGSNYNSPLHF | GNGTRLTVT | 16 | |
| HD11 | 7.1 | 1.2 | 1 | CASS | QDTGGSNYGYTF | GSGTRLTVV | 12 | |
| Vβ8 | APS12 | 8.1 | 2.3 | 2 | CAS | RPSAGADTQYF | GPGTRLTVL | 11 |
| HD2 | 8.2 | 2.7 | 2 | CASS | PRADYEQYF | GPGTRLTVT | 9 | |
| HD4 | 8.1 | 2.3 | 2 | CAS | GPSAGADTQYF | GPGTRLTVL | 11 | |
| Vβ13.1 | APS3 | 13.1 | 1.2 | 1 | CAS | ISSWTGSYGYTF | GSGTRLTVV | 12 |
| HD4 | 13.1 | 1.5 | 1 | CASS | PGTGSNQPQHF | GDGTRLSIL | 11 | |
Determined based on the nucleotide sequence of the TCRβ PCR products.
The TGxxN/Q motif in Vβ7+ and Vβ13.1+ TCRs and the PxAxxD/E motif in Vβ8+TCRs are underlined and double-underlined, respectively. Original nucleotide sequences can be obtained from GenBank database (accession number AF428125-AF428134).
The gene rearrangement and the CDR3 amino acid sequence are identical in these TCRβ chains.