The affinity of ALK-derived peptides for HLA-A*0201 molecules and immunogenicity in HHD mice
| Peptide* . | FI† . | DC50(h)‡ . | R/T HHD mice1-153 . | % lysis1-155 . |
|---|---|---|---|---|
| p280-89 | 5.6 ± 0.92 | 4 | 3/6 | 63—49—18 |
| p282-90 | 5.4 ± 1.78 | 4-6 | 3/6 | 80—56—50 |
| p376-85 | 2.8 ± 0.17 | 6 | 3/6 | 24—15—15 |
| p377-85 | 3 ± 0.35 | 4-6 | 3/6 | 48—24—21 |
| p621-29 | 3.5 ± 0.16 | 4-6 | 2/6 | 63—53 |
| p281-89 | 3.5 ± 0.65 | 2 | 2/6 | 42—34 |
| p456-65 | 4.2 ± 0.25 | 2-4 | 2/6 | 67—15 |
| p609-17 | 2.3 ± 0.35 | 2 | 0/6 | < 15 |
| p615-24 | 3.3 ± 0.14 | 2 | 0/6 | < 15 |
| Peptide* . | FI† . | DC50(h)‡ . | R/T HHD mice1-153 . | % lysis1-155 . |
|---|---|---|---|---|
| p280-89 | 5.6 ± 0.92 | 4 | 3/6 | 63—49—18 |
| p282-90 | 5.4 ± 1.78 | 4-6 | 3/6 | 80—56—50 |
| p376-85 | 2.8 ± 0.17 | 6 | 3/6 | 24—15—15 |
| p377-85 | 3 ± 0.35 | 4-6 | 3/6 | 48—24—21 |
| p621-29 | 3.5 ± 0.16 | 4-6 | 2/6 | 63—53 |
| p281-89 | 3.5 ± 0.65 | 2 | 2/6 | 42—34 |
| p456-65 | 4.2 ± 0.25 | 2-4 | 2/6 | 67—15 |
| p609-17 | 2.3 ± 0.35 | 2 | 0/6 | < 15 |
| p615-24 | 3.3 ± 0.14 | 2 | 0/6 | < 15 |
Position is referred to NPM/ALK sequence.
Ratio (median channel of fluorescence) between the sample and a control containing an irrelevant peptide. Results are expressed as the mean value of 4 experiments ± SEM.
DC50: Half-life of disassociation of HLA-A*02.01/peptide complexes.
R/T is responder versus tested mice. Mice were considered as responders when the specific lysis of peptide-loaded RMA-S/HDD target cells was at least 15% above the lysis of RMA-S/HDD.
Percent lysis of peptide-loaded RMA-S/HDD cells at an E/T ratio of 60:1. Values correspond to the maximal lysis observed for each responder mouse.