Recipients of 2 different donors can mount the recall immune response
| Responding lymph node cells . | n . | Delta CPM with different concentrations of OVA . | |||
|---|---|---|---|---|---|
| 0.01 μM . | 0.1 μM . | 1 μM . | 10 μM . | ||
| Unprimed C57BL/6 | 3 | 69 ± 81 | 133 ± 61 | 1 123 ± 104 | 8 370 ± 172 |
| Unprimed SJL/J | 3 | −104 ± 29 | −238 ± 92 | 754 ± 234 | 3 411 ± 54 |
| Primed C57BL/6 | 3 | 1 067 ± 860 | 3 356 ± 1 300 | 18 658 ± 7 988 | 41 622 ± 8 733 |
| Primed SJL/J | 3 | 57 227 ± 19 4054-150 | 85 850 ± 11 4854-150 | 126 171 ± 19 4854-150 | 134 982 ± 4 1144-150 |
| Primed BALB/c | 3 | 7 177 ± 4 565 | 14 240 ± 9 158 | 40 366 ± 11 544 | 72 703 ± 11 826 |
| C57BL/6 → BALB/c | 5 | 319 ± 119 | 752 ± 466 | 6 545 ± 4 318 | 26 591 ± 10 401 |
| SJL/J → BALB/c | 5 | 101 ± 13 | 367 ± 149 | 2 913 ± 1 159 | 21 027 ± 10 252 |
| C57BL/6 + SJL/J → BALB/c | 9 | −344 ± 660 | 253 ± 487 | 2 372 ± 954 | 17 280 ± 5 8514-151,‡ |
| Responding lymph node cells . | n . | Delta CPM with different concentrations of OVA . | |||
|---|---|---|---|---|---|
| 0.01 μM . | 0.1 μM . | 1 μM . | 10 μM . | ||
| Unprimed C57BL/6 | 3 | 69 ± 81 | 133 ± 61 | 1 123 ± 104 | 8 370 ± 172 |
| Unprimed SJL/J | 3 | −104 ± 29 | −238 ± 92 | 754 ± 234 | 3 411 ± 54 |
| Primed C57BL/6 | 3 | 1 067 ± 860 | 3 356 ± 1 300 | 18 658 ± 7 988 | 41 622 ± 8 733 |
| Primed SJL/J | 3 | 57 227 ± 19 4054-150 | 85 850 ± 11 4854-150 | 126 171 ± 19 4854-150 | 134 982 ± 4 1144-150 |
| Primed BALB/c | 3 | 7 177 ± 4 565 | 14 240 ± 9 158 | 40 366 ± 11 544 | 72 703 ± 11 826 |
| C57BL/6 → BALB/c | 5 | 319 ± 119 | 752 ± 466 | 6 545 ± 4 318 | 26 591 ± 10 401 |
| SJL/J → BALB/c | 5 | 101 ± 13 | 367 ± 149 | 2 913 ± 1 159 | 21 027 ± 10 252 |
| C57BL/6 + SJL/J → BALB/c | 9 | −344 ± 660 | 253 ± 487 | 2 372 ± 954 | 17 280 ± 5 8514-151,‡ |
At 100 days after transplantation, the mixed chimeras were immunized subcutaneously in the hind footpads with 3.5 nM whole ovalbumin emulsified in GERBU adjuvant. At 9 days later, the draining lymph node cells were harvested and cultured with various concentrations of whole ovalbumin at 5 × 105 cells per well in a 96-well flat-bottom culture plate. After 64 hours' culture, the culture was pulsed with 1 μCi 3H-thymidine per well and cultured for a further 8 hours. The cultures were harvested and counted in a β-counter. Similar experiments were repeated twice.
CPM indicates counts per minute; OVA, ovalbumin.
P < .0001, compared with primed C57BL/6 or primed SJL/J.
P < .05, compared with both unprimed and primed normal controls.
Not significant, compared with the recipients of single-donor (C57BL/6 → BALB/c or SJL/J → BALB/c) cells.