Sequence of primers used in RT-PCR assays
| Gene . | Sense primer . | Antisense primer . |
|---|---|---|
| hC/EBPε | CAGACAGGAAGGCGCTGGG | CGGCAGTGGCCAAAGGGGCCTT |
| mC/EBPε | GCTACAATCCCCTGCAGTACC | CACAAGGGCAAGAAGGCA |
| CD14 | CCCGACCCTCCAAGTTTT | ACCTGCTTCAGCCCAGTG |
| Mona | TCTGCAGCATTTCCACCA | GGAATCCCAGCTCGTCCT |
| Fcγ RI | TCGGTGGGGAAGTGGTTA | CTGGCCTCTGGGATGCTA |
| PAI-2 | TTGAGGACGCATCCACTG | GCCTTGCCCTTGTTGAAG |
| IRK1 | CTTGCCTTCGTGCTCTCC | TTGGGCACTCGTCTGTCA |
| VLA5 (α) | GCAGCTGCATTTCCGAGT | CCACAACGGGACACCATT |
| VLA5 (β) | AGCACAACCCCAGCAAAG | GATCCACAAACCGCAACC |
| IL-12p40 | TCATGGCTGGTGCAAAGA | AGGGTCTCCTCGGCAGTT |
| IL-12p35 | AGTTCCAGGCCATCAACG | AAGGCGTGAAGCAGGATG |
| IL-18 | CAGTGAACCCCAGACCAGAC | TCAGGTGGATCCATTTCCTC |
| IL-15 | CTGAGGCTGGCATTCATGT | GCAATTCCAGGAGAAAGCAG |
| IL-6 | GTGACAACCACGGCCTTC | TTCTGCAAGTGCATCATCG |
| IL-10 | TTGGGTTGCCAAGCCTTA | ACCTGCTCCACTGCCTTG |
| MCP-3 | TTCTGTGCCTGCTGCTCA | GCTTCAGCGCAGACTTCC |
| M-CSF | GCCAGACCCTCGAGTCAA | CGTGGAGGGGGAAAACTT |
| c-fms | CCGGGCTCTCAGTCTCAA | CATCTGCATGGACCGTGA |
| MCP-1 | AGCCAATCAGCCATCTGC | CTCTATGGCCTGCGGTGT |
| c-maf | AGGCGGACCCTGAAAAAC | TTTTCTCGGAAGCCGTTG |
| IL-1RA | CCCTGCCACAAACACACA | ATGTGATGCCCTGGTGGT |
| NOS | ACGCTGGCTACCAGATGC | GGGATGCTCCATGGTCAC |
| TNF-α | GTGCTCCTCACCCACACC | GGGCAGATTGACCTCAGC |
| IP-10 | ACGGGCCAGTGAGAATGA | TGTGTGCGTGGCTTCACT |
| ICSBP | ACCAAGAGGAGCCCATCC | TTCAAGGCAGGTGGTGGT |
| MR | CGGCATGGGTTTTACTGC | CACCGAAACGTCCCTTTG |
| SR type 1 | ATTGGCTTCCCTGGAGGT | CACTGGCCTTGGTGGAAG |
| SR type 2 | GGGAGTGTAGGCGGATCA | GCATGGCATGACACAGGA |
| 18S | AAACGGCTACCACATCCAAG | CCTCCAATGGATCCTCGTTA |
| Gene . | Sense primer . | Antisense primer . |
|---|---|---|
| hC/EBPε | CAGACAGGAAGGCGCTGGG | CGGCAGTGGCCAAAGGGGCCTT |
| mC/EBPε | GCTACAATCCCCTGCAGTACC | CACAAGGGCAAGAAGGCA |
| CD14 | CCCGACCCTCCAAGTTTT | ACCTGCTTCAGCCCAGTG |
| Mona | TCTGCAGCATTTCCACCA | GGAATCCCAGCTCGTCCT |
| Fcγ RI | TCGGTGGGGAAGTGGTTA | CTGGCCTCTGGGATGCTA |
| PAI-2 | TTGAGGACGCATCCACTG | GCCTTGCCCTTGTTGAAG |
| IRK1 | CTTGCCTTCGTGCTCTCC | TTGGGCACTCGTCTGTCA |
| VLA5 (α) | GCAGCTGCATTTCCGAGT | CCACAACGGGACACCATT |
| VLA5 (β) | AGCACAACCCCAGCAAAG | GATCCACAAACCGCAACC |
| IL-12p40 | TCATGGCTGGTGCAAAGA | AGGGTCTCCTCGGCAGTT |
| IL-12p35 | AGTTCCAGGCCATCAACG | AAGGCGTGAAGCAGGATG |
| IL-18 | CAGTGAACCCCAGACCAGAC | TCAGGTGGATCCATTTCCTC |
| IL-15 | CTGAGGCTGGCATTCATGT | GCAATTCCAGGAGAAAGCAG |
| IL-6 | GTGACAACCACGGCCTTC | TTCTGCAAGTGCATCATCG |
| IL-10 | TTGGGTTGCCAAGCCTTA | ACCTGCTCCACTGCCTTG |
| MCP-3 | TTCTGTGCCTGCTGCTCA | GCTTCAGCGCAGACTTCC |
| M-CSF | GCCAGACCCTCGAGTCAA | CGTGGAGGGGGAAAACTT |
| c-fms | CCGGGCTCTCAGTCTCAA | CATCTGCATGGACCGTGA |
| MCP-1 | AGCCAATCAGCCATCTGC | CTCTATGGCCTGCGGTGT |
| c-maf | AGGCGGACCCTGAAAAAC | TTTTCTCGGAAGCCGTTG |
| IL-1RA | CCCTGCCACAAACACACA | ATGTGATGCCCTGGTGGT |
| NOS | ACGCTGGCTACCAGATGC | GGGATGCTCCATGGTCAC |
| TNF-α | GTGCTCCTCACCCACACC | GGGCAGATTGACCTCAGC |
| IP-10 | ACGGGCCAGTGAGAATGA | TGTGTGCGTGGCTTCACT |
| ICSBP | ACCAAGAGGAGCCCATCC | TTCAAGGCAGGTGGTGGT |
| MR | CGGCATGGGTTTTACTGC | CACCGAAACGTCCCTTTG |
| SR type 1 | ATTGGCTTCCCTGGAGGT | CACTGGCCTTGGTGGAAG |
| SR type 2 | GGGAGTGTAGGCGGATCA | GCATGGCATGACACAGGA |
| 18S | AAACGGCTACCACATCCAAG | CCTCCAATGGATCCTCGTTA |
Mona indicates monocytic adaptor; FcγRI, Fcγ receptor type I; PAI-2, plasminogen activator inhibitor type-2; IRK1, inwardly rectifying potassium channel; VLA5, fibronectin receptor; c-fms, macrophage colony-stimulating factor receptor; IL-1RA, IL-1 receptor antagonist; NOS, nitric oxide synthase; TNF-α, tumor necrosis factor-α; IP-10, IFN-γ inducible protein 10; ICSBP, IFN consensus sequence-binding protein; MR, mannose receptor; SR, scavenger receptor.