Precision and sensitivity of intracellular IFN-γ assay and LDA
| Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | Replicate 5 | Average of the replicates | |
| Flow cytometric intracellular IFN-γ assay | ||||||
| Precision* | 3700 | 3570 | 3570 | 3220 | 3220 | 3456 |
| Sensitivity† | ||||||
| Concentration | 1:2 | 1:4 | 1:8 | 1:16 | 1:160 | |
| Results | 1840 | 1470 | 700 | 460 | 40 | |
| 1780 | 1320 | 820 | 380 | 90 | ||
| 2000 | 1240 | 830 | 360 | 60 | ||
| Average of the triplicates | 1870 | 1340 | 780 | 400 | 60 | |
| Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | Replicate 5 | Average of the replicates | |
| Limiting-dilution analysis | ||||||
| Precision‡ | 11 | 4 | 10 | 6 | 1.7 | 4.4 |
| Sensitivity2-153 | ||||||
| Concentration | 1:2 | 1:4 | 1:8 | 1:16 | 1:160 | |
| Results | 1.2 | 0.8 | ND | ND | ND | |
| Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | Replicate 5 | Average of the replicates | |
| Flow cytometric intracellular IFN-γ assay | ||||||
| Precision* | 3700 | 3570 | 3570 | 3220 | 3220 | 3456 |
| Sensitivity† | ||||||
| Concentration | 1:2 | 1:4 | 1:8 | 1:16 | 1:160 | |
| Results | 1840 | 1470 | 700 | 460 | 40 | |
| 1780 | 1320 | 820 | 380 | 90 | ||
| 2000 | 1240 | 830 | 360 | 60 | ||
| Average of the triplicates | 1870 | 1340 | 780 | 400 | 60 | |
| Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | Replicate 5 | Average of the replicates | |
| Limiting-dilution analysis | ||||||
| Precision‡ | 11 | 4 | 10 | 6 | 1.7 | 4.4 |
| Sensitivity2-153 | ||||||
| Concentration | 1:2 | 1:4 | 1:8 | 1:16 | 1:160 | |
| Results | 1.2 | 0.8 | ND | ND | ND | |
Five independent samples were analyzed to determine the precision of the intracellular IFN-γ assay and LDA, as described in “Materials and methods.” Sensitivity was determined by analyzing samples after serial dilution of the T cells, as described in “Materials and methods.” Results represent absolute numbers of EBV-specific T cells per 105 T lymphocytes.
ND indicates not detectable.
Results of 5 replicates of the undiluted concentration are shown.
The results and average of triplicates for the titrations 1:2 to 1:160 are shown.
Results of 5 replicates of the undiluted concentration are shown.
The results for the titrations 1:2 to 1:160 are shown.